Thermo Fisher, N8080246, AmpliTaq Gold™ DNA Polymerase with Buffer I

Name: AmpliTaq Gold™ DNA Polymerase with Buffer I
Brand: Thermo Fisher
SKU: N8080246

Quantity: 1, 000 units, Unit Size: Each, AmpliTaq Gold DNA Polymerase with Buffer I is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation. The modified enzyme is provided in an inactive state and upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

Catalog Number: N8080246

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Category

Hot Start Polymerases

Pack Size000 units / pack

TagsPCR Equipment and Supplies PCR Supplies Standard PCR Products Hot Start PCR Reagents and Kits

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 1,000 units
Unit Size: Each
AmpliTaq Gold DNA Polymerase can be activated partially or completely in a pre-PCR heat step or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases as reactants are not wasted in the formation of unintended products.Features
• Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
• Time-released thermal activation improves sensitivity in low copy number amplifications
• Successful multiplex PCR saves time and reagents
• Includes GeneAmp 10X PCR Buffer I with 15 mM MgCl_2
• Hot-start technology reduces risk of biological contaminationNotes
• For better performance AmpliTaq Gold 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation.
• When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates.

Specifications

General

Exonuclease Activity	5' - 3'
Fidelity (vs. Taq)	1X
Format	Tube
Hot Start	Built-In Hot Start
No. of Reactions	800 Reactions
Overhang	3'-A
Polymerase	AmpliTaq Gold DNA Polymerase
Product Type	DNA Polymerase With Buffer I
Quantity	1,000 units
Reaction Format	Separate Components
Shipping Condition	Dry Ice
Size (Final Product)	5 kb or less
Starting Material	DNA
Concentration	5 U/μL
For Use With (Application)	Hot-start PCR
GC-Rich PCR Performance	Low
Reaction Speed	Standard
Unit Size	Each

Ordering

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Unless otherwise stated, the minimum order amount is USD 1,000.
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