Description
Quantity: 1 kit
Unit Size: Each
Qdot™ streptavidin conjugates consist of a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Qdot™ nanocrystal). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays, and are also used in purification workflows to achieve targeted fractionation. Each of the six conjugates in the Qdot™ Streptavidin Sampler Kit is supplied as a 1μM solution (50μL volume).Important Features of the Qdot™ Streptavidin Sampler Kit:
• Streptavidin Qdot™ conjugates have emission maxima of ∼525, ∼565, ∼585, ∼605, ∼655, and ∼705 nm
• Approximately 5 to 10 streptavidins per Qdot™ nanocrystal
• Extremely photostable and bright fluorescence
• Efficiently excited with single-line excitation sources
• Narrow emission, large Stokes shift
• Available in multiple colors
• Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and moreProperties of Qdot™ NanocrystalsThe Qdot™ streptavidin conjugate is the size of a large macromolecule or protein (∼15–20 nm) and represents the brightest class of streptavidin detection reagents. Qdot™ streptavidin conjugates are made from a nanometer-scale crystal of a semiconductor material (CdSe), which is coated with an additional semiconductor shell (ZnS) to improve the optical properties of the material. The Qdot™ 705 and Qdot™ 800 streptavidin conjugates, which include CdSeTe, are made in a similar fashion. This core-shell material is further coated with a polymer shell that allows the materials to be conjugated to biological molecules and to retain their optical properties.Other Fluorescent Conjugates of Streptavidin are AvailableWe offer several otherQdot™ colorsand wide range of streptavidins conjugated to Alexa Fluor™ dyes, Oregon Green™ dye, enzyme conjugates, and traditional fluorophores like Texas Red™ dye, fluorescein (FITC) andmore.Find Biotinylated ConjugatesWe offer an extensive line of biotinylated conjugates for use in biotin-streptavidin detection strategies.
• Use theprimary antibody search toolto find biotinylated primary antibodies
• Use thesecondary antibody selector toolto find biotinylated secondary antibodies and biotinylated anti-dye and anti-hapten antibodiesBlocking Endogenous BiotinNaturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try ourEndogenous Biotin-Blocking Kitto minimize this interference.For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.Related Links:Learn more about Avidin-Biotin DetectionTo learn more about Qdot™ Nanocrystals
