Thermo Fisher, R01688, Porphyrin Test Agar

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Porphyrin Test Agar is a solid medium that is used for speciation of Haemophilus based on the ability to convert delta-aminolevulinic acid (ALA) to porphyrin. Porphyrin Test Agar contains hemoglobin as a source of hemin. Porphyrin Test Agar contains GCHI enrichment, a defined supplement which provide NAD, vitamins, amino acids, coenzymes, dextrose, and other nutrients necessary for the growth of Haemophilus spp. This medium provides an accurate means to determine X factor requirement based on the observation that X-independent Haemophilus strains excrete porphobilinogen and porphyrins, all of which are intermediates in the hemin biosynthetic pathway when supplied with ALA. After incubation there is a color change in the colonies in presence of longwave ultraviolet light.