Thermo Fisher, R061170, Indole Broth (4mL)

Quantity: 100 x 4 mL
Unit Size: Each
Microorganisms that possess the enzyme tryptophanase have the ability to hydrolyze and deaminate tryptophan with the production of indole, pyruvic acid and ammonia^3. Indole that splits from the tryptophan molecule on addition of the reagent ρ-dimethylaminobenzaldehyde results in a distinct red color that can be easily detected^4.
• Ready to use- Liquid media
• Recommended by APHA in theIdentification of Enterobacteriaceaefor use in the differentiation of enteric gram-negative rods^1,2.
• Easy to interpret-Positive test - Pink to red colored ring at the interface of the broth and the reagentNegative test - No color developmentIndole Broth contains casein peptone that supplies essential growth factors, trace elements, and is the source of tryptophan in this medium.. Sodium chloride maintains osmotic equilibrium. Certain gram-negative rods release indole after hydrolyzing tryptophan. After addition of dimethylaminobenzaldehyde, it combines with the indole and produces a distinct red color.