Description
Quantity: 0.75 mL
Unit Size: 0.75 mL
Transfecting stem cells without inhibiting cell viability and cell growth can be challenging due to the sensitivity of these cells. Lipofectamine Stem is a low toxicity reagent, requiring low amounts of nucleic acid, allowing your stem cells to stay healthy and continue proliferating without inducing differentiation. Lipofectamine Stem reagent is:
• Superior in performance—up to 3-fold higher efficiency across iPSCs, hESCs, NSCs, and MSCs compared to other reagents
• Versatile—one reagent for DNA, RNA, Cas9 protein, and co-transfection
• Gentle on cells—maintains cell proliferation without inducing differentiation
• Flexible—transfects cells in adherence or suspension—an alternative to electroporationAchieve high efficiency gene editing in stem cellsGene editing applications typically require the ability to co-deliver DNA, RNA, and/or protein into cells. Lipofectamine Stem Transfection Reagent is designed to co-deliver CRISPR/Cas9 complexes into human stem cells for high efficiency gene editing. This one reagent can be used to transfect large DNA plasmids such as all-in-one CRISPR/Cas9 vectors and co-deliver a range of RNAs, such as large Cas9 mRNA with tracr/crRNA complexes or single guide (sg) RNA, and protein such as Cas9 protein combined with tracr/crRNA or sgRNA.A low-toxicity alternative to electroporationTransfecting stem cells via electroporation methods can be challenging and cumbersome. Electroporation protocols recommend single cell suspension culture to ensure maximum efficiency and minimal toxicity. Using the reverse transfection protocol, the Lipofectamine Stem Transfection Reagent proves to be an effective and less toxic alternative to electroporation for stem cells. It requires low amounts of nucleic acid to achieve maximum transfection efficiency, on par with electroporation, while supporting continued proliferation without inducing differentiation.
