Thermo Fisher, X14210, X-Rhod-1, AM, cell permeant - Special Packaging

Quantity: 10 x 50 μg
Unit Size: 10 x 50 µg
• Uses in many calcium signaling investigations, including measuring Ca^2+in cells and tissues
• Cells may be loaded with the AM ester forms of these calcium indicators by adding the dissolved indicator directly to dishes containing cultured cells
• Fluorescence signal from these cells is generally measured using fluorescence microscopy
• Exhibit fluorescence increase upon binding Ca^2+with little shift in wavelength
• In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn^2+, Zn^2+, Pb^2+) with substantially higher affinity than Ca^2+
• Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelator
• Label (Ex/Em of Ca^2+–bound form): X-rhod-1 (580/602nm)
• Fluorescence intensity increase upon binding Ca^2+: >100 fold
• K_dfor Ca^2+in the absence of Mg^2+, in buffer: approximately 700nMCalcium Detection, Cell Analysis, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling