The Waters Lipid Conjugated ASO LC-MS Standard is a 5 nmole quantity of a 16 residue gapmer antisense oligonucleotide (ASO) containing a fully phosphorothioated backbone, a 5’ palmitate modification and methoxy ethyl modified termini. Its sequence is provided in its manual and on its certificate of analysis. It has a negative control sequence that does not have any homology with mammalian genes. The 5’ modification is created from the use of a 5'-Palmitate-C6 CE phosphoramidite. This is a standard that is to be used as a performance check and potential system suitability standard for oligonucleotide extractions with the OligoWorks SPE Kits as well as LC-UV and LC-MS analyses, as performed with IP-RPLC, HILIC or IEX separations.
| Separation Mode | Reversed Phase |
|---|---|
| Detection Method | MS |
| Formulation | Solid |
| Storage Condition | -20C +/- 10C (Freezer) |
| System Type | LC/MS |
| Instrument | UPLC, UHPLC, HPLC |
| Shelf Life | 24 Months |
| Shelf life upon reconstitution | 1 Week |
| Shipping Condition | Ambient |
| Tolerance | +/- 20% |
| Type | System Performance |
| UNSPSC | 41116107 |
| Application | Oligonucleotide, Gene Therapeutics |
| Brand | Waters |
| Product Type | Standards |
| Units per Package | 1 pk |
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