BHB00S04-1046WT, YMC-BioPro HIC

High-Performance Columns for Protein Separation Based on Hydrophobicity
Hydrophobic Interaction Chromatography (HIC) separates biomolecules based on differences in surface hydrophobicity under mild, non-denaturing conditions. This technique is particularly valuable for the purification and characterization of proteins, antibodies, fragments, and variants , maintaining biological activity throughout the process.
In HIC, target molecules bind to mildly hydrophobic ligands on the stationary phase in the presence of high salt concentrations. As the salt concentration is gradually decreased, bound molecules elute according to their degree of hydrophobicity, offering a selective, scalable approach for biopharmaceutical purification.
YMC’s BioPro HIC series delivers exceptional resolution, recovery, and reproducibility across analytical and preparative workflows.