Description
Key facts
Sample types:Urine, Plasma, Tissue Extracts, Cell culture media, Serum, Other biological fluids, Cell Lysate,
Assay type:Quantitative,
Sensitivity:~ 60 µM,
Range:2 - 15 nM,
Assay time:40m,
Assay Platform:Microplate reader
Product details:
Magnesium Assay Kit (ab102506) is a simple sensitive assay for quantitating magnesium in a variety of biological samples. The kit takes advantage of the specific requirement of glycerol kinase for Mg2+. An enzyme linked reaction leads to formation of an intensely colored (λmax = 450nm) product whose formation is proportional to Mg2+ concentration.
The linear range of the assay is 2-15 nmoles with detection sensitivity ~40 μM.
Other Notes
This product was previously called K385 Biovision Magnesium Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.
Magnesium is the 11th most abundant element by mass in the human body. Mg2+ is essential to all living cells where it plays an important role in facilitating the processing of biological polyphosphates like ATP, DNA, RNA and enzyme functions. Mg2+ is the metallic ion at the center of chlorophyll, and a common additive to fertilizers. Mg2+ compounds are used as laxatives, antacids, and used to stabilize abnormal nerve excitation and blood vessel spasm i.e., eclampsia.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Magnesium is an essential mineral with a significant role in numerous enzymatic reactions. It weighs approximately 24.305 atomic mass units. This mineral is expressed throughout the body particularly in bones muscles and soft tissues. The concentration of magnesium fluctuates based on dietary intake cellular demand and transport mechanisms. Magnesium ion (Mg2+) actively participates in biochemical reactions by acting as a cofactor which often stabilizes phosphate groups in ATP and other nucleotides.
Biological function summary
Magnesium contributes to cellular energy production DNA synthesis and protein synthesis. It is integral to hundreds of enzyme systems within the human body. Magnesium is not directly part of a complex but influences many enzyme systems and cellular processes. Its influence on excitability is noteworthy as it plays a central role in nerve impulse transmission and muscular contraction regulation.
Pathways
Magnesium influences several key biological pathways including the Krebs cycle and oxidative phosphorylation. Within these pathways magnesium interacts with various enzymes and proteins such as ATP synthase. Additionally magnesium plays a regulatory role in maintaining homeostasis within these systems affecting calcium and sodium ion concentrations.
Magnesium deficiency or imbalance relates to cardiovascular diseases and migraines. Cardiovascular health can be influenced by magnesium's relationship with the proteins involved in ion transport such as sodium-potassium pumps. In migraines magnesium interacts with neurotransmitter receptors and influences pathways that impact pain reception and transmission. These processes illustrate the critical importance of magnesium in maintaining health and preventing disease.
