Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture media, Cell culture supernatant, Serum, Other biological fluids,
Assay type:Enzyme activity,
Sensitivity:= 0.01 mU/well,
Assay time:40m,
Assay Platform:Microplate reader
Product details:
Alcohol Dehydrogenase Assay Kit ab102533 provides a convenient tool for sensitive detection of the Alcohol dehydrogenase (ADH) activity in a variety of samples.
Alcohol dehydrogenase assay principle
In the assay, alcohol dehydrogenase acts on ethanol, a chemically coupled reaction with a probe results in a colorimetric (450 nm) readout. This assay detects ADH activity as low as 0.01 mU in samples.
Alcohol dehydrogenase assay protocol summary
- add samples and standards to wells
- add reaction mix
- incubate for 3 min
- analyze with microplate reader, incubate for 30 min to 2 hrs and analyze again
Other notes
This product was previously called K787 Biovision Alcohol Dehydrogenase Activity Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.
Alcohol dehydrogenase (Alcohol DH, ADH) (EC 1.1.1.1) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alcohol dehydrogenase (ADH) also known as aldehyde reductase is an enzyme that catalyzes the conversion of alcohols to aldehydes while reducing NAD+ to NADH. This enzyme's structure comprises approximately 40 kDa per subunit and it typically functions as a dimer. ADH is predominantly expressed in the liver where it plays a major role in alcohol metabolism. However its presence is also noted in other tissues such as the stomach lining and kidneys. The enzyme's activities are significant enough to be measured through various assays including ADH assay and alcohol dehydrogenase test highlighting its enzymatic kinetics.
Biological function summary
Alcohol dehydrogenase's role extends beyond simple alcohol metabolism. It participates in the body's detoxification processes. This enzyme is not part of a larger complex but can function alongside other dehydrogenases involved in metabolic processes. ADH's activity helps in managing blood alcohol levels by converting ethanol to acetaldehyde which is further processed by aldehyde dehydrogenase to acetate. The balance and efficiency of these reactions determine the rate and capacity of alcohol clearance from the body.
Pathways
Alcohol dehydrogenase significantly contributes to the alcohol metabolism pathway and the retinol metabolism pathway. It plays a role in converting retinol to retinal which is important for vision and cell growth. In these pathways ADH works closely with aldehyde dehydrogenase to ensure the continuation of the metabolic processes in the human body. The enzyme's function is also connected to the smooth operation of the cytochrome P450 system in the liver for comprehensive detoxification.
The activity of alcohol dehydrogenase links closely to conditions like alcoholism and liver disease. Variations in ADH enzyme activity can affect an individual's susceptibility to alcohol use disorder due to differences in alcohol metabolism rates. Additionally high acetaldehyde levels a product of the alcohol dehydrogenase reaction can contribute to liver damage leading to cirrhosis. This enzyme's relationship to aldehyde dehydrogenase is critical as the failure in converting acetaldehyde can exacerbate the toxic effects and contribute further to these disorders.
