Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture extracts, Tissue Extracts, Serum, Other biological fluids,
Assay type:Quantitative,
Assay time:20m,
Assay Platform:Microplate reader
Product details:
Bradford Assay Kit ab102535 provides a simple and rapid procedure for determining the concentration of protein in solution.
The Bradford assay protocol uses an improved Coomassie blue G reagent which forms a blue complex in the presence of protein. The intensity of the blue complex is proportional to the amount of protein in the sample and can be easily measured by spectrophotometer or plate reader at 595 nm.
Bradford assay protocol summary:
- prepare serial dilutions of protein standard
- add samples and standards to wells or tubes
- add Bradford protein assay solution and incubate for 5 min at room temp
- measure absorbance at 595 nm
Support on how to determine protein concentration can be found
This product is manufactured by BioVision, an Abcam company and was previously called K810 Protein Quantitation Kit. K810-1000 is the same size as the 1000 test size of ab102535.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Protein quantitation is an essential process used to determine the concentration of proteins in a sample primarily employing methods like the Bradford assay. This target involves the interaction of proteins with specific reagents such as the Bradford reagent which causes a colorimetric change quantifiable by spectrophotometry. The Bradford assay protein protocol is popular due to its simplicity and sensitivity utilizing a standard curve created with known concentrations of Bovine Serum Albumin (BSA). This methodology estimates protein concentrations in various samples from tissues or cellular extracts where proteins range in mass significantly and influence diverse biological processes.
Biological function summary
Protein quantitation supports critical cellular activities by enabling the study and understanding of proteins involved in diverse cellular mechanisms. In many complexes proteins have various roles such as structural enzymatic or regulatory directly affecting cell function and response to environmental changes. The Bradford test is frequently used to analyze proteins in research settings facilitating studies on cellular signaling transport and metabolic regulation. Proper protein quantitation ensures accurate downstream analysis like Western blotting or enzyme activity assays important for elucidating protein function and interaction.
Pathways
Protein quantitation provides insights into metabolic and signaling pathways by accurately measuring protein levels and monitoring changes due to external stimuli or genetic manipulation. This assay assists in exploring pathways such as the MAPK/ERK signaling cascade and the ubiquitin-proteasome system which coordinate cell growth differentiation and apoptosis. Proteins like kinases and ubiquitin ligases are often investigated using protein quantitation methods to understand better how these pathways function and contribute to cell homeostasis.
Protein quantitation aligns closely with research on conditions like cancer and neurodegenerative diseases. Changes in protein expression can be indicative of disease progression or therapeutic response making accurate quantitation essential. For instance protein quantitation helps identify alterations in levels of proteins such as p53 in cancer or amyloid-beta in Alzheimer's disease. By understanding these protein changes researchers can design better diagnostic tools and treatments potentially improving patient outcomes.
