Description
Product details:
PE/Cy5.5® Conjugation Kit - Lightning-Link® (ab102899) uses a simple and quick process for PE/Cy5.5 labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides.
To conjugate an antibody to PE/Cy5.5® using this kit:
- add modifier to antibody and incubate for 3 hrs
- add quencher and incubate for 30 mins
The PE/Cy5.5® conjugated antibody can be used immediately in Flow cytometry and other applications. No further purification is required and 100% of the antibody is recovered for use.
Learn about our antibody labeling kits and their advantages
Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our
antibody purification and concentration kits
Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.
Amount and volume of antibody for conjugation to PE/Cy5.5
Kit size
Recommended
amount of antibody
Maximum antibody
volume
3 x 10 µg
3 x 10 µg
3 x 10 µL
1 x 60 µg
1 x 60 µL
3 x 60 µg
3 x 60 µg
3 x 60 µL
600 µg
1 x 600 µg
1 x 600 µL
Ideal antibody concentration is 1mg/mL. 0.5 - 1 mg/mL can be used if the maximum antibody volume is not exceeded. Antibodies > 1 mg/mL or < 0.5 mg/mL should be diluted /concentrated.
The selling size of this product has been changed – it is now based on the amount of antibody that can be conjugated with the kit, not the amount of PE mix provided. The amount of antibody advised that can be used with the kit has also been updated to reflect what will give the best conjugation results. The quantity and formulation of reagents provided have not changed, if you have been previously using the kit successfully with a different amount of antibody, there is no need to change the way that you are using the kit.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.
50mM / 0.6% Tris
0.1% BSA
50% glycerol
0.1% sodium azide
Potassium phosphate
Sodium chloride
Sucrose
Sodium citrate
Trehalose
Tris buffered saline is almost always ≤ 50 mM / 0.6%
Incompatible buffer constituents
Thiomerosal
Proclin
Glycine
Arginine
Glutathione
If a constituent of the buffer containing your antibody or protein is not listed above, please contact us.
Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.
Storing and handling conjugation kits
Lyophilized Lightning-Link
components are hygroscopic.
Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.
This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link
R-PE/Cy5.5 Labeling Kit. 761-0005 is the same as the 60 µg size. 761-0010 is the same as the 3 x 60 µg size. 761-0030 is the same as the 3 x 10 µg size. 761-0015 is the same as the 600 µg size.
Properties and Storage Information:
Shipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
