Description
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Urine,
Reacts with:Mammals,
Assay type:Competitive,
Sensitivity:= 8.68 pg/mL,
Range:20 - 2000 pg/mL,
Assay Platform:Microplate
Product details:
17 beta Estradiol ELISA Kit ab108667 is a competitive ELISA kit to measure quantify 17-beta-estradiol (E2) with a sensitivity of 8.68 pg/mL.
How the assay works
17 beta Estradiol ELISA Kit ab108667 is a competitive ELISA. There are 2 types of competitive ELISAs:
antigen down
: The sample antigen competes with a reference antigen for binding to a specific amount of labeled antibody. The reference antigen is pre-coated on a multi-well plate. The sample is added to the plate wells with a labeled antibody. Depending on the amount of antigen in the sample, more or fewer free antibodies will be available to bind the reference antigen. This means that the more of the antigen there is in the sample, the less reference antigen will be detected and the weaker the signal will be.
antibody down
: the labeled antigen and the sample antigen (unlabeled) compete for binding to the primary antibody, which is immobilized on the plate. The lower the amount of antigen in the sample, the stronger the signal due to more labelled antigen in the well.
Assay specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility.
17 beta Estradiol ELISA Kit ab108667 protocol summary
1. A 96-well plate is provided in the kit, that has been precoated with anti-Estradiol IgG.
2. Samples and the Estradiol-HRP conjugate are added to the wells, where Estradiol in the sample competes with the added Estradiol-HRP for antibody binding.
3. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration.
4. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow.
5. The intensity of signal is inversely proportional to the amount of Estradiol in the sample and the intensity is measured at 450 nm.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Estradiol 17-beta also known as 17-beta estradiol or beta estradiol is a steroid hormone with a molecular weight of approximately 272.38 g/mol. This hormone is the most biologically active form of estrogen and primarily synthesized by the ovaries though its presence is also noted in adipose tissue and the adrenal glands. Within the body 17-beta estradiol travels through the bloodstream to various tissues where it binds to estrogen receptors modulating gene expression and affecting numerous physiological processes.
Biological function summary
Estrogenic activities of estradiol 17-beta are important for reproductive and non-reproductive systems. It significantly influences the development and maintenance of female secondary sexual characteristics. This hormone also engages in the regulation of the menstrual cycle and reproductive function by interacting with other hormonal signals in the body. It can form complexes with estrogen receptors leading to DNA interactions that regulate the transcription of certain genes. Beyond reproduction estradiol 17-beta exerts effects on bone density cardiovascular health skin health and brain function.
Pathways
Various cellular systems utilize estradiol 17-beta in their signaling. It takes an essential role in the estrogen signaling pathway and interacts with the estrogen receptor pathway. These interactions commonly involve crosstalk with other proteins such as progesterone and androgens impacting cellular functions. The estrogen signaling pathway influence on gene transcription highlights its part in regulating cell proliferation apoptosis and differentiation processes.
Hormone imbalances involving estradiol 17-beta can lead to various conditions. It is notably associated with breast cancer where abnormal levels and receptor engagement can stimulate tumor growth. Osteoporosis is another disorder linked to estrogen levels as estradiol 17-beta preserves bone density by modulating bone resorption. Additionally hormonal fluctuations involving related proteins like aromatase and sex hormone-binding globulin (SHBG) can exacerbate the progression of these diseases further linking estradiol 17-beta to critical health outcomes.
