Description
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Indirect,
Assay Platform:Microplate
Product details:
Human Anti-Cytomegalovirus IgG ELISA Kit (CMV) is an indirect ELISA kit for the qualitative detection of human IgG antibodies against CMV with a 98% sensitivity.
How the assay works
Human Anti-Cytomegalovirus IgG ELISA Kit (CMV)ab108724 is an indirect ELISA kit.
A 96-well plate has been precoated with Cytomegalovirus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Cytomegalovirus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Cytomegalovirus IgG sample captured in plate.
Assay specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility.
Human Anti-Cytomegalovirus IgG ELISA Kit (CMV)ab108724 protocol summary
1. Add controls and samples to appropriate wells - Incubate for 1hr
2. Wash wells
3. add anti-cytomegalovirus IgG-HRP conjugate - Incubate for 30 min
4. Wash
5. add TMB substrate - Incubate for 15 min
6. Add Stop solution. Read at 450 nm within 30 min.
How other researchers are using Human Anti-Cytomegalovirus IgG ELISA Kit (CMV) ab108724
Human Anti-Cytomegalovirus IgG ELISA Kit (CMV)ab108724 has been used to assess the effect of CMV seropositivity on ChAdOx1 vaccination immunogenicity
and to study immunoageing in T-cell compartments
References
HR Sharpe et.al. PMID 35192547
Ni Zeng et al. PMID 35037711
Related and Recommended products
Other ELISA kits can be used to assess CMV serostatus together with Human Anti-Cytomegalovirus IgG ELISA Kit (CMV)ab108724:
- Human Anti-Cytomegalovirus IgM ELISA Kit (CMV)
ab108725
Epstein-Barr virus (EBV) is another common herpesvirus causing similar symptoms. ELISA kits to assess EBV serostatus are:
- Human Anti-Epstein Barr virus IgG ELISA Kit (EBV-VCA)
ab108730
- Human Anti-Epstein Barr virus IgG ELISA Kit (EBV-EBNA)
ab108731
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
