Description
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Indirect,
Assay Platform:Microplate
Product details:
Abcam's anti-Mycoplasma pneumoniae IgA Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgA class antibodies against Mycoplasma pneumoniae in Human serum and plasma.
A 96-well plate has been precoated with Mycoplasma pneumoniae antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgA conjugate is added to the wells, which binds to the immobilized Mycoplasma pneumoniae-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Mycoplasma pneumoniae IgA sample captured in plate.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Mycoplasma pneumoniae IgA is an immunoglobulin A (IgA) antibody targeting the Mycoplasma pneumoniae bacterium. This protein does not have an alternate common name. Its mass is typically not measured as a standalone protein due to the variability in IgA glycoproteins. IgA expression occurs mainly in mucosal areas including respiratory and gastrointestinal mucosa as it plays an important role in mucosal immunity. Mycoplasma pneumoniae is a small bacterium lacking a cell wall capable of causing respiratory infections particularly in enclosed community settings such as schools.
Biological function summary
The immune system utilizes Mycoplasma pneumoniae IgA to prevent colonization by this bacterium and to neutralize its effect. While not forming part of a large multi-protein complex IgA engages with various immune elements assisting in pathogen clearance. This antibody contributes to immune response modulation and mucosal protection stopping the Mycoplasma pneumoniae bacterial infections from impeding normal physiological function.
Pathways
Interactions involving Mycoplasma pneumoniae IgA align with mucosal immunity and adaptive immune pathways. In these pathways this antibody operates in concert with other immunoglobulins such as IgM and IgG to establish an effective line of defense against bacterial invaders. These immune pathways facilitate the identification and removal of Mycoplasma pneumoniae maintaining respiratory health and preventing persistent infection.
Mycoplasma pneumoniae IgA is strongly connected to respiratory diseases especially atypical pneumonia or walking pneumonia. In this context elevated levels of specific IgA can indicate infection aiding in diagnosis. Furthermore this marker has associations with extrapulmonary manifestations of infection such as skin rashes and joint inflammation. These disease processes may involve other proteins like pro-inflammatory cytokines highlighting IgA’s role in both respiratory and systemic immune responses.
