Description
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Indirect,
Assay Platform:Microplate
Product details:
Abcam's anti-Parainfluenza virus 1, 2, 3 IgG Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Parainfluenza virus 1, 2, 3 in Human serum and plasma.
A 96-well plate has been precoated with Parainfluenza virus 1, 2, 3 antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Parainfluenza virus 1, 2, 3-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Parainfluenza virus 1, 2, 3 IgG sample captured in plate.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Parainfluenza Virus IgG interacts mechanically with the antigens of human parainfluenza viruses (HPIV) including parainfluenza 1 2 3 and 4 signaling an immune response. This IgG detects viral proteins expressed on the surface of infected host cells serving as a marker of past exposure or infection. The molecular mass of the parainfluenza virus proteins varies but the surface glycoproteins typically studied are Hemagglutinin-neuraminidase (HN) and Fusion (F) proteins. Parainfluenza viruses are expressed in the respiratory tract cells of infected individuals enabling the spread of viral particles.
Biological function summary
The parainfluenza virus IgG participates in immune surveillance and viral neutralization. It plays a significant role in adaptive immunity by recognizing viral epitopes on the HN and F proteins blocking receptor binding and aiding in virus agglutination. This IgG is not part of an active complex but collaborates with other immune components such as T-cells and complement proteins enhancing the clearance of infected cells.
Pathways
The recognition by parainfluenza virus IgG engages the antiviral immune pathways essential for protecting respiratory tissues. It functions within the humoral immune response pathway alongside other proteins like IgM and IgA to prevent viral spread. In the adaptive immune response it also interacts with memory B cells ensuring long-term immunological memory against future parainfluenza infections.
Parainfluenza virus IgG connects closely to respiratory illnesses primarily croup and bronchiolitis often affecting young children. Variations in IgG response can influence the severity and recurrence of these infections. This target associates with proteins like Interferon-gamma and Interleukin-6 which play roles in modulating inflammation and virus clearance in the body. This connection to the immune response further emphasizes its role in disease resolution and potential complications arising from immune imbalances.
