Description
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Indirect,
Assay Platform:Microplate
Product details:
Abcam's anti-Respiratory syncytial virus (RSV) IgM Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Respiratory syncytial virus in Human serum and plasma.
A 96-well plate has been precoated with Respiratory syncytial virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Respiratory syncytial virus -specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Respiratory syncytial virus IgM sample captured in plate.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Respiratory Syncytial Virus (RSV) IgM is an immunoglobulin M antibody produced in response to RSV infection. IgM antibodies are among the first antibodies to be produced during the initial immune response. The mass of an individual IgM molecule can range around 900 kDa due to its pentameric structure. RSV-specific IgM is expressed early in infections mainly in the plasma cells derived from B lymphocytes. IgM detection can signify an acute or recent infection with RSV which makes it a valuable target in diagnostic testing scenarios.
Biological function summary
The immunoglobulin M targeting RSV plays a vital role in the acute immune response by facilitating early viral neutralization and assisting in the phagocytosis of viral particles. It does not participate in protein complexes but acts independently as an antibody in the serum. The timely production of RSV IgM helps initiate the primary immune response which creates a defense mechanism against the virus before more specific and longer-lasting antibodies like IgG come into play.
Pathways
The production of RSV IgM is part of the humoral immune response pathway particularly important in the early stages of immunity. This pathway involves the activation of B cells and their differentiation into plasma cells that produce antibodies. Two important proteins in this context are CD40 which stimulates B cells and interleukin-6 (IL-6) which promotes antibody production. These elements work together in the immune response to create a rapid defense against RSV.
RSV IgM has a significant connection to respiratory illnesses such as bronchiolitis and pneumonia particularly in infants and young children. The presence of RSV-specific IgM can indicate acute RSV infection. Besides its response can be altered in patients with immunodeficiencies. No specific proteins are consistently associated with the disease process linked to RSV that directly interact with IgM but a dysregulated immune response involving cytokines or other immune modulators can influence the severity of RSV-induced illness.
