Description
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Indirect,
Sensitivity:= 0.45 U/mL,
Range:10 - 100 U/mL,
Assay Platform:Microplate
Product details:
Abcam's anti-Rubella virus IgG Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Rubella virus in Human serum and plasma.
A 96-well plate has been precoated with Rubella virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Rubella virus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Rubella virus IgG sample captured in plate.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rubella Virus IgG also known as anti-rubella IgG represents a group of antibodies directed against the rubella virus. These antibodies are important for identifying and assessing immune response to the rubella virus infection. They are predominantly found in the blood serum of individuals who have been vaccinated against rubella or who have had a rubella virus infection. Anti-rubella IgG antibodies offer reliable evidence of immunity and long-term protection against the virus. Typically no standard mass applies to IgG antibodies as they can vary slightly between individuals. These antibodies are mainly expressed in response to an immune challenge specifically from the rubella virus.
Biological function summary
Anti-rubella IgG antibodies play a significant role in neutralizing the rubella virus and preventing its replication. These antibodies do not act as part of a larger protein complex but instead bind directly to antigens on the virus surface. If the antibodies demonstrate high avidity they indicate a mature immune response which could be significant in determining the timing of rubella infection. A high avidity index often suggests past infection or vaccination providing valuable information about the rubella immunity status. Therefore measuring the avidity of anti-rubella IgG can help distinguish between recent and past infections.
Pathways
The immune response pathways involve these antibodies as a part of the adaptive immune response. Key pathways include the humoral immune response where B cells produce specific antibodies against rubella after recognizing the virus antigens. Other proteins such as CD4+ T-helper cells assist B cells in differentiating into plasma cells that secrete these antibodies. This process strengthens the body's defense mechanisms by equipping it with memory against future rubella exposures.
Rubella virus IgG antibodies are especially pivotal in the context of congenital rubella syndrome (CRS) which can occur when a pregnant woman contracts rubella virus. Detecting these antibodies in pregnant women can assess immunity and risk of CRS. Additionally they might relate to autoimmune disorders where the enhanced immune activity could trigger autoantibody production. Here connections with proteins like rheumatoid factor which is often elevated in autoimmune conditions might be observed reflecting the immune system's broader response dynamics.
