Description
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Indirect,
Assay Platform:Microplate
Product details:
Abcam's anti-Schistosoma mansoni IgG Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Schistosoma mansoni in Human serum and plasma.
A 96-well plate has been precoated with Schistosoma mansoni antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidise (HRP) labelled Protein A conjugate is added to the wells, which binds to the immobilized Schistosoma mansoni-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Schistosoma mansoni IgG sample captured in plate.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Schistosoma mansoni IgG also known as anti-Schistosoma IgG is an immunoglobulin targeting the parasite Schistosoma mansoni. This antibody plays a significant role in the immune response against the parasitic infection. The molecular mass of IgG generally ranges around 150 kDa. It is primarily expressed in the blood by B cells as part of the immune system response to parasitic infections. Detection of Schistosoma mansoni IgG is often utilized in diagnostic tests providing insights into exposure and immune response levels.
Biological function summary
This immunoglobulin facilitates the recognition and neutralization of the Schistosoma mansoni parasite. It does not form part of large protein complexes but interacts intricately with antigen-binding regions. Upon binding IgG initiates a cascade of immune responses including opsonization which marks the parasite for destruction by other immune cells. This antibody functions as an important component in targeting and managing parasitic infection through its specific affinity for schistosome antigens.
Pathways
Schistosoma mansoni IgG plays a pivotal role in the adaptive immune response against parasitic infections. It is integral to the antibody-mediated immune pathways including the complement activation pathway and antibody-dependent cellular cytotoxicity. These pathways involve other proteins such as complement proteins which enhance the ability of antibodies to clear pathogens. IgG's involvement assists in neutralizing the parasite and mitigating the infection's impact.
Schistosoma mansoni IgG is directly related to schistosomiasis also known as bilharzia. The presence and levels of this antibody in serum serve as indicators of exposure and an ongoing immune response to Schistosoma mansoni infection. Its detection is important for diagnosing schistosomiasis. Additionally proteins such as the parasite's antigens play a role in the disease process interacting with IgG to trigger an immune response that helps control the infection.
