Description
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Indirect,
Sensitivity:< 1 IU/mL,
Range:1 - 200 U/mL,
Assay Platform:Microplate
Product details:
Abcam's anti-Toxoplasma gondii IgG Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Toxoplasma gondii in Human serum and plasma.
A 96-well plate has been precoated with Toxoplasma gondii antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Toxoplasma gondii-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Toxoplasma gondii IgG sample captured in plate.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Toxoplasma gondii IgG also known as anti-Toxoplasma IgG is an antibody that plays a significant role in the immune response against the protozoan parasite Toxoplasma gondii. This immunoglobulin has a molecular mass of approximately 150 kDa and is primarily expressed in the bloodstream during Toxoplasma infection. The detection of Toxoplasma gondii IgG serves as an important marker in serological tests used to determine past infection or exposure to this parasite.
Biological function summary
Toxoplasma gondii IgG helps mediate the immune response by binding to the surface antigens of the parasite aiding in its identification and destruction by immune cells. This interaction is facilitated by the high avidity of the IgG enhancing its ability to remain bound to Toxoplasma antigens under challenging conditions. Toxoplasma gondii IgG is not part of a larger protein complex but instead operates as a standalone molecule in the immune defense system.
Pathways
Toxoplasma gondii IgG operates within the adaptive immune pathway a critical component of immune system functioning. This pathway involves other proteins such as major histocompatibility complex (MHC) molecules and T-cell receptors which play roles in antigen presentation and cellular immune response. The interplay of these molecular components enables the immune system to effectively target and respond to Toxoplasma gondii.
Toxoplasma gondii IgG is primarily associated with toxoplasmosis an infectious disease caused by Toxoplasma gondii. In immunocompromised individuals or during pregnancy this infection can lead to severe complications. During disease progression IgG levels are often measured as part of diagnosis and monitoring. Additionally research has explored its role in the context of congenital toxoplasmosis where IgG avidity tests aid in differentiating recent infections from past ones providing insights into disease management.
