Description
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Indirect,
Assay Platform:Microplate
Product details:
Abcam's anti-Trichinella spiralis IgG Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Trichinella spiralis in Human serum and plasma.
A 96-well plate has been precoated with Trichinella spiralis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Trichinella spiralis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Trichinella spiralis IgG sample captured in plate.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Trichinella spiralis IgG is an important antibody for detecting Trichinella spiralis a parasitic nematode responsible for trichinellosis in humans. Alternative names include anti-Trichinella spiralis antibody. The IgG class of antibodies has a typical mass around 150 kDa as is common with most IgG antibodies. It is primarily expressed in the serum of infected individuals where it participates in immune responses against the parasite. As part of the host's adaptive immunity these antibodies bind to specific antigens on the surface of the Trichinella spiralis larvae marking them for destruction by immune cells.
Biological function summary
The role of Trichinella spiralis IgG involves recognition and neutralization of the parasitic nematodes in the host organism. While not part of a multi-protein complex itself IgG antibodies operate through a network of immune interactions enhancing phagocytosis by macrophages and neutrophils via opsonization. This immune response reduces parasitic loads and assists in containing the spread of infection within tissues such as muscle where the larvae encyst. As with other IgG antibodies they initiate complement activation further promoting parasite elimination.
Pathways
The activity of Trichinella spiralis IgG is integral to both the classical and the alternative complement pathways. This antibody interacts with the complement system proteins such as C1q which start the cascade leading to the formation of the membrane attack complex. The regulation of humoral immune responses involves Fc receptors on various immune cells which recognize the Fc region of IgG. Proteins like C4 and C3 in the complement pathway relate with the functional activities driven by these antibodies facilitating pathogen lysis and clearance.
Trichinella spiralis IgG antibodies are primarily associated with the diagnosis and progression of trichinellosis characterized by gastrointestinal disturbances and muscle pain. During infection the body's immune response to the parasite can cause inflammation and tissue damage leading to symptoms. Interestingly cross-reactivity with other helminth infections might occur due to shared antigenic features which could involve proteins like helminth-derived proteases. Accurate detection of specific IgG antibodies helps in confirming diagnosis and distinguishing trichinellosis from other parasitic infections that exhibit similar clinical signs.
