Description
Key facts
Detection method:Colorimetric,
Sample types:Tissue, Cerebral Spinal Fluid, Saliva, Urine, Cell culture supernatant, Milk, Cell Lysate,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.89 ng/mL,
Range:3.125 - 200 ng/mL,
Assay time:3h,
Assay Platform:Microplate
Product details:
Human Albumin ELISA Kit ab108788 is a sandwich ELISA to measure Human Albumin (ALB) in Tissue, Cerebral Spinal Fluid (CSF), Saliva, Urine, Cell culture supernatant, Milk, Cell Lysate with a sensitivity of 0.79 ng/mL.
How the assay works
An albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of albumin captured in plate.
Human Albumin ELISA Kit ab108788 protocol summary
1. Add samples or standards to the wells
2. Incubate for 1 hour then wash
3. Add biotinylated detector antibody
4. Incubate for 30 min then wash
5. Add the SP-conjugate
6. Incubate for 30 min then wash
7. Add the chromogen substrate
8. Incubate for 25 min
9. Add stop solution and read at 450 nm
How other researchers are using Human Albumin ELISA kit ab108788
The Human Albumin ELISA kit ab108788 has been used in publications to assess therapeutic effects of adipose-derived stem cells on acute liver failure
, effects of GP130 signaling in liver stenosis
, and potential therapeutic targets for diabetic nephropathy
References:
Xiaoyan Zhang et al. 2023, PMID: 37078751,
Zhe Li et al. 2023 PMID: 37058568,
Liyu He et al. 2022, PMID: 35813614
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- Human Albumin ELISA Kit
ab179887
Related and recommended products
Human Albumin ELISA kit ab108788 is commonly used by researchers to measure liver or kidney function.
Liver function ELISA assays include Human ALT ELISA kit
ab234578
and Human AST ELISA Kit
ab263881
These ELISA kits are also available for Mouse/Rat:
- Mouse Albumin ELISA kit
ab207620
- Mouse ALT ELISA kit
ab282882
- Mouse AST ELISA kit
ab263882
- Rat Albumin ELISA kit
ab235642
- Rat ALT ELISA kit abab234579
- Rat AST ELISA kit
ab263883
Kidney function marker assays include:
- Creatinine Assay Kit
ab65340
– suitable for use as a serum creatinine assay and a urine creatinine assay.
- Albumin (BCG) Assay Kit (Colorimetric)
ab235628
– suitable for use with serum
- Urea Assay Kit
ab83362
– suitable for use with serum/plasma and urine, and used as a blood urea nitrogen assay.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.
Biological function summary
Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.
Pathways
More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.
Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.
