Abcam, ab111622-55mL, Janus Green cell normalization stain

Key facts
Applications:In-Cell ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Form:LiquidSee storage information,
Storage buffer:Constituents: Water, 0.81% Sodium chloride, 0.3% Janus Green B, 0.216% Disodium hydrogenorthophosphate, 0.02% Potassium chloride, 0.02% Potassium phosphate monobasic

Product details:
Ready-to-use green stain for normalizing antibody signal intensity to total cell amounts during in-cell ELISA (ab111622).
Cells fixed to a solid surface (typically in microplate wells) can be stained by adding 1X Janus Green Stain. Incubate cells for 5 minutes at room temperature. Dye should be removed and washed 5 times in ultrapure water or until excess dye is removed. The last wash should be removed and add 0.1 mL 0.5 M HCl per well and incubate for 10 minutes. Shake the plate for 10 seconds, and measure the OD 595 nm using a standard microplate spectrophotometer.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C