Description
Key facts
Detection method:Colorimetric,
Sample types:Saliva, Plasma, Cell culture extracts, Suspension cells, Adherent cells,
Assay type:Quantitative,
Assay Platform:Microplate reader
Product details:
Aldehyde Assay Kit (Colorimetric) ab112113 is a a sensitive, one-step colorimetric assay to detect as little as 1 nanomole of aldehyde in a 100 μL assay volume (10 μM). The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and readily adapted to automation without a separation step.
The aldehyde assay protocol uses a proprietary dye that generates a chromogenic product upon reacting with an aldehyde. Most of the existing aldehyde test methods are based on separations either by the tedious and expensive HPLC-MS or GC-MS.
The signal is read with an absorbance microplate reader at 405 or 550 nm.
Aldehyde assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30-60 min
- analyze with a microplate reader
Previously called Aldehyde Quantification Assay Kit (Colorimetric).
This kit has been used for monitoring activities of oxidases that convert an amino group to an aldehyde group.
ab112113 should be stored desiccated.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Aldehydes play an important role in various biological processes. Also known as aliphatic aldehydes or aromatic aldehydes when describing specific types aldehydes can be seen throughout different environments in the human body. These small organic compounds possess a molecular mass that varies depending on the specific type of aldehyde but they generally consist of a carbonyl center with a hydrogen atom single-bonded to the carbon atom making them relatively lightweight molecules. Aldehydes are highly reactive and widely distributed in several tissues and organs such as the liver where they are heavily expressed due to the organ's metabolic functions.
Biological function summary
Aldehydes do several critical tasks in the body. They are involved in metabolic processes such as energy production and detoxification. Aldehydes can also act as signaling molecules to influence various pathways. Aldehyde dehydrogenase enzymes often act in conjunction with aldehydes transforming them into less reactive carboxylic acids. This metabolic action highlights their role as part of larger multistep enzymatic pathways demonstrating their involvement in cellular metabolism and chemical detoxification processes.
Pathways
Aldehydes participate in critical metabolic pathways including gluconeogenesis and ethanol metabolism. In the ethanol metabolism pathway the enzyme alcohol dehydrogenase initially converts ethanol into acetaldehyde which aldehyde dehydrogenase then oxidizes to acetic acid. This pathway plays a role in both energy production and cellular detoxification. Aldehydes also interact with proteins such as cytochrome P450 enzymes which are key components of drug metabolism pathways assisting in the processing of endogenous and exogenous compounds.
Aldehydes have been associated with conditions like alcohol-related liver disease and Alzheimer's disease. Acetaldehyde a type of aldehyde is implicated in the toxicity of alcohol consumption leading to liver damage when accumulated excessively. Elevated levels of aldehydes have also been detected in neurodegenerative conditions; they can contribute to oxidative stress and neuronal damage. Proteins such as amyloid-beta in Alzheimer's disease are influenced by aldehydes which can exacerbate pathological states. Understanding aldehyde function provides insight into both normal physiology and these disease mechanisms.
