Description
Key facts
Assay time:1h,
Sample types:Tissue, Suspension cells, Adherent cells
Product details:
Nuclear Extraction Kit (ab113474) provides a simple and selective method along with all necessary reagents for nuclear protein extraction / nuclear protein fractionation in just 1 hour.
The extracts can then be used in western blotting, protein-DNA binding assays, nuclear enzyme assays or any other procedures requiring optimized nuclear proteins. The protocol is fast and easy-to-use, and isolates very abundant yields of nuclear extract from mammalian cells or tissue samples.
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Compared to other kits that use conventional nuclear extraction / nuclear fractionation methods, the buffers included in ab113474 contain much lower amounts of salts (80% less than conventional kits) and no SDS, which allows much better retention of enzyme activity in the nuclear extracts.
The 1M DTT included in this kit may precipitate if exposed to temperatures below the recommended storage range. While the kit should be stored at 4°C, temperatures lower than this -such as those sometimes found in lab refrigerators- can lead to precipitation.
If precipitation occurs, we recommend re-dissolving the DTT by gently shaking the vial in a 37°C water bath until fully dissolved.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
