Description
Key facts
Applications:In-Cell ELISA, ELISA, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Form:LiquidSee storage information,
Storage buffer:Preservative: 100% Casein Blocking Buffer 10x
Product details:
Protein blocking buffer (pH 7.5) (ab126587) for serum-free blocking of non-specific antibody binding in ICC, ELISA and western blot. BSA-free
Preparation:
Dilute to 1X in deionized water or PBS (e.g. 1mL 10X blocking buffer + 9mL water/PBS). Diluting in PBS instead of water makes a somewhat more stringent blocking buffer due to the presence of salt in the PBS. Blocking Buffer is pH 7.5. The user should determine empirically whether dilution in water or PBS is most appropriate for the assay at hand. Use diluted block solution promptly.
Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient, Storage information-The product can be stored for up to 12 months
