Key facts
CAS number:93839-89-5,
Purity:>80%,
Form:SolidSee storage information,
Molecular weight:547.1 Da,
Molecular formula:C10H12Li4N5O12P3S,
PubChem:5311341,
Nature:Synthetic,
Solubility:Soluble in water to 10 mM,
Biochemical name:Adenosine 5'-(trihydrogen diphosphate), monoanhydride with phosphorothioic acid, tetralithium salt,
Biological description:Substrate and inhibitor of ATP-dependent enzyme systems. Hydrolyzed very slowly by phosphatases and most ATPases. Once thiophosphorylated, proteins are resistant to protein phosphatases. P2 purinergic receptor agonist.,
Canonical smiles:[Li+].[Li+].[Li+].[Li+].C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)([O-])OP(=O)([O-])OP(=S)([O-])[O-])O)O)N,
Isomeric smiles:[Li+].[Li+].[Li+].[Li+].C1=NC(=C2C(=N1)N(C=N2)[C@H]3[C@@H]([C@@H]([C@H](O3)COP(=O)([O-])OP(=O)([O-])OP(=S)([O-])[O-])O)O)N,
InChi:InChI=1S/C10H16N5O12P3S.4Li/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(25-10)1-24-28(18,19)26-29(20,21)27-30(22,23)31;;;;/h2-4,6-7,10,16-17H,1H2,(H,18,19)(H,20,21)(H2,11,12,13)(H2,22,23,31);;;;/q;4*+1/p-4/t4-,6-,7-,10-;;;;/m1..../s1,
InChiKey:DWQFDOIBOYDYKH-KWIZKVQNSA-J,
IUPAC Name:tetralithium;[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-oxidophosphoryl] dioxidophosphinothioyl phosphate
Product details:
Kinase reaction reagent to be used with
ab92570
rabbit monoclonal to thiophosphate ester for the identification of direct kinase substrates as mentioned in Allen JJ
et al.
Nat Methods 4:511-6 (2007).
After the kinase of interest has accepted ATP-γ-S,
-Nitrobenzyl mesylate (ab138910) can be used to alkylate the thiophospholyation site on the substrates. A thiophosphate ester rabbit monoclonal antibody (ab92570) is introduced to identify the tagged substrates.
For western blot analysis, we used ATPγS or A*TPγS analogs at a concentration of 1 mM. For kinetic measurements, ATPγS or A*TPγS analog concentration varied from 0.1 μM to 250 μM. See Allen JJ
et al.
Nat Methods 4:511-6 (2007); Supp. Material.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Store under desiccating conditions|The product can be stored for up to 12 months
