Description
Key facts
Detection method:Fluorescent,
Sample types:Suspension cells, Adherent cells,
Assay type:Cell-based,
Assay Platform:Flow cytometer, Fluorescence microscope
Product details:
Abcam's Aggresome Detection kit (ab139486) provides a rapid, specific and quantitative approach to identify inhibitors relevant to neurodegenerative disease in an authentic cellular context. This fixed-cell assay doesn't require non-physiological protein mutations or genetically engineered cell lines. The Aggresome Detection Reagent has been validated under a wide range of conditions and with small molecule modulators, demonstrating suitability for screening compounds of potential therapeutic value, and optimized for antibody co-localization studies to identify interactions between aggregated protein cargo and the various proteins implicated in autophagy and aggresome formation. Suggested uses for this kit include:
1. The identification of small molecules that inhibit aggresome formation.
2. Immuno-localization studies between aggregated protein cargo and the various proteins implicated in aggresome formation, such as histone deacetylase 6, parkin, ataxin-3, dynein motor complex and ubiquilin-1.
Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information--80°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The aggresome is a cellular structure that functions in the degradation of misfolded proteins. Often considered an organized response to proteotoxic stress the aggresome acts as a storage site for misfolded proteins to facilitate their clearance. Cells typically express aggresomes in response to an overwhelmed proteasome system. While their actual molecular weight varies due to the complex and aggregate nature they often form near the centrosome of mammalian cells near the microtubule-organizing center.
Biological function summary
The aggresome participates in protein quality control within the cell. This complex serves an essential role in cellular homeostasis by sequestering misfolded or aggregated proteins for degradation through autophagy. Once these misfolded proteins get directed to the aggresome cells envelop them in autophagosomes which facilitates their eventual breakdown in lysosomes. The presence of ubiquitin chains often tags proteins targeted for aggresome formation implicating the ubiquitin-proteasome system in this process.
Pathways
Aggresome formation plays a significant role in the cellular stress response. The ubiquitin-proteasome pathway heavily influences this process given its role in tagging proteins for degradation. Aggresomes collaborate with chaperone proteins like heat shock proteins to recognize and transport misfolded proteins. In addition they interface with the autophagic pathway which links aggresomes to LAMP proteins found on lysosomal membranes that mediate protein degradation within lysosomes.
The aggresome formation holds considerable relevance to neurodegenerative diseases like Alzheimer's and Parkinson's. In these conditions protein aggregation becomes a hallmark feature with aggresome components sometimes linked to disease pathogenesis. For instance α-synuclein in Parkinson’s disease can form aggregates that contribute to neurodegeneration. Similarly the protein tau in Alzheimer's disease can aggregate disrupting neuronal function. Aggresome targeting involves these proteins highlighting the critical intersection of protein management and neurodegenerative disease pathology.
