Abcam, ab145248-5g, DAN, Fluorescence determination of NO2-
MW 158.2 g/mol, Purity >98%. Routinely used in the determination of nitrite/nitrate levels in biological fluids and cellular extracts as one indicator of nitric oxide activity. The assay uses the reaction of DAN with NO 2 - under acidic conditions to form a detectable fluorescent naphthotriazole. The reaction proceeds at acidic pH at room temperature. Fluorescence is monitored following the addition of NaOH, which raises pH, resulting in lower background and increased sensitivity. Also used as a
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Catalog Number: ab145248-5g
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Pack Size5 g
