Abcam, ab14943-500µg, 10X His tag® peptide

Key facts
Purity:>90% HPLC,
Tags:Tag free,
Applications:BLSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Animal free:No,
Carrier free:No

Product details:
HIS-TAG® is a trademark of EMD Biosciences, Inc.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn't dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions--20°C