Description
Key facts
Sample types:Tissue sections
Product details:
Giemsa Stain Kit (May-Grunwald) ab150670 is intended for use in the visualization of cells present in hematopoietic tissues and certain microorganisms. This kit may be used on formalin-fixed, paraffin-embedded or frozen sections.
Giemsa staining protocol summary:
- deparaffinize sections if necessary and rehydrate
- incubate with May-Grunwald solution for 5-7 min
- wash with PBS
- incubate with Giemsa solution for 10-15 min
- wash with PBS, and incubate in PBS for 5 min
- rinse with water and air dry
- clear with Xylene or similar, and mount
Other products for staining tissue sections
Find more kits and reagents in the
special stains guide
, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the
IHC kits and reagents guide
Staining Interpretation
Nuclei
Blue/Violet
Cytoplasm
Light Blue
Collagen
Pale Pink
Muscle Fibre
Pale Pink
Erythrocytes
Gray, Yellow or Pink
Rickettsia
Reddish-Purple
Helicobacter Pylori
Mast Cells
Dark Blue with Red Granules
Control Tissue:
Blood Film. Any well fixed tissue.
Other Notes
Please note, the 500 mL selling size, was previously described as 100 Test. There has been no change to the physical product.
Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient, Storage information-Ambient
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Hematopoietic cells are integral components of the blood system responsible for the formation of all blood cells including red and white blood cells and platelets. These cells are primarily found in the bone marrow and express markers such as CD34 contributing to their distinction from other cell types. Microorganisms especially bacteria and parasitic organisms like Plasmodium species interact with these hematopoietic cells during infection processes. Giemsa stain and its variants such as May-Grünwald stain and Giemsa stain kit frequently serve to initially identify and characterize these interactions within blood smears.
Biological function summary
Hematopoietic cells contribute to both regenerative processes and immune responses. They form part of the hematopoietic lineage and associate with several cellular complexes that regulate proliferation differentiation and apoptosis. Bacteria and parasites attach to these cells exploiting them for survival and replication. The use of Giemsa staining as with the May-Grünwald Giemsa method allows scientists to observe these cellular structures and pathogens with clarity. Giemsa stain colors the cells to reveal distinct features useful in pathological diagnostics.
Pathways
Hematopoietic cells engage in pathways such as the JAK-STAT and TLR signaling pathways critical for hematopoiesis and immune modulation. Hematopoietic cells partner with proteins like JAK2 and TLR4 which facilitate the signaling processes essential for cell survival and immune responses. Giemsa staining aids in studying these pathways by enabling the visualization of morphological changes in blood cells as they undergo differentiation or respond to infections.
Hematopoietic cells and their interactions with microorganisms relate closely to conditions such as acute myeloid leukemia and malaria. Mutations in hematopoietic cells can lead to disorders like leukemia involving proteins such as FLT3. Malaria a parasitic infection often involves disruptions to hematopoietic cell populations with Plasmodium pathogens visible under Giemsa staining. Understanding these pathways and interactions through the use of stains supports the diagnosis and treatment of such diseases.
