Description
Key facts
Sample types:Tissue sections,
Assay time:45m
Product details:
How the assay works
In Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) protocol, tissue carbohydrates are oxidized by periodic acid forming aldehydes capable of binding with Schiff’s Solution. Visualization of Schiff’s is caused by a restoration of the dye’s quinoid structure resulting in characteristic magenta staining. Light green provides a contrasting counterstain. The staining pattern of the lymphocytes are helpful in making therapeutic decisions in established cases of lymphocytic leukemia.
Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) protocol summary:
- deparaffinize sections (if necessary) and hydrate
- immerse slide in PAS solution for 5-10 min
- rinse 4 times with water
- immerse slide in Schiff's solution for 15-30 min and rinse
- stain slide with Hematoxylin for 2-3 min and rinse
- incubate in Bluing reagent for 30 s and rinse
- incubate in Light Green Solution for 2 min and rinse
- dehydrate with alcohol, clear and mount
Staining Interpretation
Fungal Organisms Magenta
PAS Positive Material Magenta
Nuclei Black/Blue
Other Tissue Components Green/Blue
Control Tissue: Kidney, Intestine, Liver.
For the demonstration of fungal organisms in tissue sections, we recommend PAS Stain Kit (Fungus, Periodic Acid Schiff Stain)
ab245886
How other researchers are using
Periodic Acid Schiff (PAS) Stain Kit (Mucin Stain) has been used in a variety of sample type including:
- Mouse Liver tissues 1
- Mouse kidney tissues 2
- Human cornea 3
References:
1 - Liu Z et al. 2023; 2 - Meng Q et al. 2023; 3 - Kim H et al. 2023.
Related and recommended products
Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Other Notes
Please note, the 125 mL selling size, was previously described as 100 Test. There has been no change to the physical product.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Lymphocytes are critical cells of the immune system known for their roles in adaptive immunity. They express a variety of receptors including T-cell receptors and B-cell receptors on their surface which allow them to recognize antigens. These receptors have different masses depending on the specific type. Lymphocytes primarily reside in the blood lymphoid organs like the spleen and lymph nodes and mucosal tissues. Mucopolysaccharides also known as glycosaminoglycans are long chains of sugar molecules found throughout the body including on cell surfaces and in the extracellular matrix. They vary in mass depending on their structure and their main role is to provide structural support and help in cell signaling.
Biological function summary
Lymphocytes play essential roles in the immune response by recognizing and attacking foreign invaders such as viruses and bacteria. They are not part of a larger complex but function through interactions with other immune cells. Mucopolysaccharides contribute to maintaining the structural integrity and fluidity of tissues. They support cell adhesion and communication create hydrophilic environments and are involved in cell migration. In histology PAS staining is a common technique to identify mucopolysaccharides as they stain positively highlighting the presence of these molecules as PAS positive material due to the PAS stain color.
Pathways
Lymphocytes are central to immune system pathways particularly the adaptive immune response and the lymphoid signaling pathway. For the adaptive immune response they interact with proteins such as CD4 and CD8 essential in T-cell function. In the lymphoid signaling pathway they transmit signals that lead to immune activation and regulation. Mucopolysaccharides participate in signaling pathways like those involving transforming growth factor-beta (TGF-beta) where they mediate cell growth and differentiation.
Lymphocytes are a focus in conditions such as autoimmune diseases and lymphomas. Aberrations in their function can result in autoimmune disorders where the immune system attacks self-tissues. Lymphomas involve malignant transformation of lymphocytes leading to cancer of the lymphatic system. In these contexts proteins such as interleukins and tumor necrosis factor are relevant. Mucopolysaccharides are involved in disorders like mucopolysaccharidoses a group of metabolic disorders caused by enzyme deficiencies leading to the accumulation of these compounds. Proteins such as enzymes responsible for their breakdown like iduronate sulfatase are closely connected in the metabolic pathways disrupted in these diseases.
