Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Serum, Cell Lysate, Tissue Homogenate, Tissue Lysate,
Assay type:Enzyme activity (quantitative),
Sensitivity:< 0.1 mU/well,
Assay time:30m,
Assay Platform:Microplate reader
Product details:
ALDH Activity Assay Kit (Colorimetric) (ab155893) is a simple, fast and reliable method to quantify ALDH enzymatic activity
ALDH assay principle
In the ALDH assay, acetaldehyde is oxidized by ALDH generating NADH which then reduces a colorless probe to a colored product with strong absorbance at 450 nm.
ALDH assay protocol summary
- Add samples and standards to wells
- Add reaction mix and incubate for 20-60 min
- Analyze with a microplate reader at 5 min and 20-60 min
Related and recommended products
For fluorometric detection, we recommend ALDH Activity Assay Kit (Fluorometric)
ab155894
Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit
ab115348
uses antibody-pulldown to specifically measure the activity of mitochondrial ALDH2.
Other notes
This product was previously called K731 Biovision Aldehyde Dehydrogenase Activity Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.
The NAD-dependent Aldehyde Dehydrogenase (ALDH) plays a vital role in cellular detoxification. It oxidizes various aldehydes and generates the corresponding carboxyolic acid. ALDH have been found in every cellular compartment. Based on its structure and function, ALDH comprises 3 major classes in mammals: Class 1 and Class 3 (the tumor form) are located in the cytosol and include both constitutive and induced forms; Class 2 is located in the mitochondria and only exists as the constitutive form. In humans, the ALDH superfamily consists of 19 genes. The mutation of ALDH genes (loss of function) causes human diseases such as Type II hyperprolinemia, pyridoxine-dependent seizure and hyperammonemia. Recent studies show that increased ALDH activity leads to several types of malignancies, serves as a cancer stem cell marker and correlates with poor prognosis.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Aldehyde dehydrogenase often called ALDH is an enzyme family that plays an important mechanical role in cellular metabolism by oxidizing aldehydes into carboxylic acids. The molecular mass of ALDH enzymes varies but they are generally around 50-56 kDa. ALDH is mainly expressed in the liver as well as in other tissues such as the kidney and the stomach. The enzyme is known for its role in detoxifying alcohol-derived acetaldehyde a toxic compound therefore protecting the cells from oxidative stress.
Biological function summary
Aldehyde dehydrogenase enzymes significantly contribute to metabolic processes by helping the conversion of aldehydes. ALDH is part of a complex network of enzymes involved in cellular homeostasis. This family of enzymes influences the regulation of cell proliferation and differentiation due to its metabolic function. The ability of ALDH to maintain low levels of reactive aldehydes is important for many cellular activities linking these enzymes to protective and regulatory roles within the cell.
Pathways
Aldehyde dehydrogenase enzymes are integral to alcohol metabolism and the detoxification pathway in humans. They play a role in the oxidizing step of ethanol metabolism to prevent the accumulation of acetaldehyde. Additionally ALDH enzymes interact with related proteins such as alcohol dehydrogenase (ADH) which converts ethanol into acetaldehyde. This interaction in the metabolic pathway keeps toxic levels of aldehyde in check and supports overall cellular metabolism and detoxification pathways.
Altered expression or function of aldehyde dehydrogenase has links to alcoholism and certain cancers. Insufficient ALDH activity may lead to alcohol intolerance or liver disease due to buildup of toxic acetaldehyde. In cancer ALDH expression serves as a marker for cancer stem cells in various types of tumors. In both cases ALDH interactions with proteins such as acetaldehyde dehydrogenase and related metabolic enzymes play a role in the pathogenesis or progression of these conditions.
