Description
Key facts
Detection method:Fluorescent,
Sample types:Plasma, Cell culture supernatant, Serum, Cell Lysate, Tissue Culture Media,
Assay type:Enzyme activity (quantitative),
Sensitivity:< 0.05 mU/well,
Assay Platform:Microplate reader
Product details:
Abcam's PicoProbe ALDH Activity Assay Kit (ab155894) is a robust tool to quantify ALDH (aldehyde dehydrogenase) enzymatic activity. In this assay, acetaldehyde is oxidized by ALDH to form the NADH which couples with the PicoProbe to generate a potent fluorescence (Ex/Em = 535/587). The ALDH fluorometric assay kit is 10 times more sensitive than the ALDH colorimetric assay and can detect < 0.05 mU ALDH activity (based on our unit definition) in a variety of samples.
This product is manufactured by BioVision, an Abcam company and was previously called K741 PicoProbe™ Aldehyde Dehydrogenase Activity Fluorometric Assay Kit. K741-100 is the same size as the 100 test size of ab155894.
The NAD-dependent Aldehyde Dehydrogenase (ALDH) plays a vital role in cellular detoxification. It oxidizes various aldehydes and generates the corresponding carboxyolic acid. ALDH have been found in every cellular compartment. Based on its structure and function, ALDH comprises 3 major classes in mammals: Class 1 and Class 3 (the tumor form) are located in the cytosol and include both constitutive and induced forms; Class 2 is located in the mitochondria and only exists as the constitutive form. In humans, the ALDH superfamily consists of 19 genes. The mutation of ALDH genes (loss of function) causes human diseases such as Type II hyperprolinemia, pyridoxine-dependent seizure and hyperammonemia. Recent studies show that increased ALDH activity leads to several types of malignancies, serves as a cancer stem cell marker and correlates with poor prognosis.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Aldehyde Dehydrogenase (ALDH) an important enzyme responsible for oxidizing aldehydes into carboxylic acids participates in detoxifying aldehydes produced during various metabolic processes. ALDH has several isoforms including the well-studied ALDH2 which possesses a molecular mass of approximately 56 kDa. ALDH2 is found in many tissues but highest levels are in liver and kidney playing a significant role in processing endogenous aldehydes and those derived from external sources such as alcohol metabolism.
Biological function summary
Aldehyde Dehydrogenase influences the metabolism of aldehydrates aiding in the protection of cells from aldehyde-induced stress. ALDH exists within a larger family of enzymes and does not typically operate as part of a large complex. It helps maintain the balance of reactive aldehyde species in the cell through oxidation facilitating cellular protection against oxidative damage.
Pathways
Aldehyde Dehydrogenase plays an integral part in alcohol metabolism and the larger detoxification pathways. The enzyme ALDH2 is notably involved in the ethanol degradation pathway where it metabolizes acetaldehyde to acetate a less toxic compound. It interacts closely with alcohol dehydrogenase which initially forms acetaldehyde from ethanol establishing a sequential relationship within this critical metabolic pathway.
ALDH2's role is closely linked with alcoholism and Asian flush syndrome. ALDH2 deficiency impairs acetaldehyde detoxification leading to accumulation resulting in facial flushing and increased risk of alcoholism-related complications. Interaction with other proteins such as cytochrome P450 enzymes can impact the severity of these conditions as they are involved in overlapping detoxification pathways that handle a variety of substrates and drugs.
