Description
Key facts
Detection method:Colorimetric,
Sample types:Nuclear Extracts, Purified protein,
Assay type:Sandwich,
Sensitivity:> 1 ng,
Range:500 - 20000 ng,
Assay time:3h 30m,
Assay Platform:Microplate reader
Product details:
The Universal SIRT Activity Assay Kit (Colorimetric) is a complete set of optimized buffers and reagents for measuring the activity/inhibition of total SIRT enzymes using nuclear extracts or purified SIRT isoforms (SIRTs 1-7) from a broad range of species such as mammals, plants, fungi, and bacteria, in a variety of forms including, but not limited to cultured cells and fresh and frozen tissues. Detection of inhibition or activation of SIRTs is important in elucidating mechanisms of epigenetic regulation of gene activation and silencing and may benefit diagnostics and therapeutics of cancer or neurological diseases. The Universal SIRT Activity Assay Kit (Colorimetric) provides the components to successfully achieve this.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SIRT1 also known as Silent mating type information regulation 2 homolog 1 is a NAD-dependent deacetylase enzyme. SIRT1 weighs approximately 120 kDa and plays an important role in regulating transcription apoptosis and stress resistance. Researchers have found SIRT1 in various tissues with higher expression in the heart brain and skeletal muscle. It is a component of the larger family of sirtuins which are involved in metabolic regulation and aging.
Biological function summary
SIRT1 modulates several cellular processes such as gene silencing DNA repair and lifespan extension. SIRT1 participates in complexes with other proteins including histones and transcription factors to influence chromatin structure and gene expression. It acts through deacetylation of target proteins affecting their function and stability. The activity of SIRT1 is also linked to environmental and cellular conditions including caloric intake and oxidative stress.
Pathways
SIRT1 is integral in the regulation of metabolic and longevity pathways. It interacts with the FOXO family proteins and the tumor suppressor protein p53 aiding in response to cellular stress and metabolic demands. The role of SIRT1 in the insulin signaling pathway exemplifies its influence on glucose homeostasis and energy balance. These interactions highlight its importance in metabolic health and aging.
SIRT1 links to neurodegenerative diseases such as Alzheimer's disease and metabolic disorders like type 2 diabetes. In Alzheimer's disease SIRT1 interacts with the amyloid precursor protein suggesting a protective role against amyloid-beta accumulation. Additionally studies have shown connections between SIRT1 and insulin receptor substrates highlighting its role in managing insulin sensitivity and glucose metabolism in diabetes. Understanding SIRT1's functions offers potential therapeutic targets for these disorders.
