Description
Product details:
HeLa cells are an adherent human epithelial cell line derived from a cervical carcinoma. HeLa cells were lysed with 1% SDS in the prescence of protease and phosphatase inhibitors. Protein concentration of the extract was determined by BCA assay. The extract is suitable for use in SDS-PAGE and Western blotting.
PMA-treated HeLa lysate is designed for use as a western blot positive control when studying signaling cascades downstream of PKC. Cells were treated with 200 nM PMA for 1 hour after overnight serum starvation. Untreated cells were grown under the same conditions and treated instead with DMSO (PMA diluent as used to generate the treated lysate).
Concentration: HeLa PMA-treated lysate, 200 μg at 2.0 mg/mL
HeLa DMSO-treated lysate, 200 μg at 2.0 mg/mL
PMA (phorbol 12-myristate 13-acetate) also known as TPA (12-O-Tetradecanoylphorbol 13-acetate), is a potent activator of protein kinase C (PKC), a calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase. PKC is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP.
Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information--80°C
