Description
Key facts
Detection method:Colorimetric,
Sample types:Tissue Lysate, Cell Lysate,
Assay type:Sandwich (quantitative),
Sensitivity:= 2.8 pg/mL,
Range:18.8 - 1200 pg/mL,
Assay time:1h 30m,
Assay Platform:Microplate
Product details:
GFP ELISA Kit ab171581 is a rapid single-wash 90-min ELISA kit to measure GFP (Green Fluorescence Protein) in tissue lysates and cell lysates. This SimpleStep ELISA® sensitivity is 2.8 pg/mL.
How the assay works
GFP (Green Fluorescence Protein) SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit has been validated to detect both the Enhanced and Superfold variants of GFP.
GFP ELISA Kit ab171581 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using GFP ELISA Kit ab171581:
GFP ELISA Kit ab171581 has been used to visualize tauopathy in Alzheimer's disease
, to study adeno-associated viral vectors for human gene transfer
, and to analyze protein secretions from Parkinson's Disease patients
References:
Marcia N Gordon et al. 2023, PMID: 36970910,
Catherine O'Riordan et al. 2020, PMID: 32578442,
Bruno Manadas et al. 2020, PMID: 32266064
Save your precious samples
Our 384-well plate GFP ELISA Kit only requires a maximum of 12.5µL
Related and recommended products
GFP ELISA Kit ab171581 is commonly used to study gene expression and protein interactions. GFP is a fluroscent protein used for labeling and visualizing cellular processes.
Other ELISA kits for labeling and visualization include:
- mCherry ELISA kit
ab221829
Secondary antibodies for labeling and visualization include:
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)
ab150077
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)
ab150113
Green fluorescent protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technology has considerably contributed to a greater understanding of cellular physiology.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GFP also known as Green Fluorescent Protein acts as a bioluminescent marker derived from the jellyfish Aequorea victoria. GFP is popular in molecular biology for its fluorescence properties making it useful for visualizing proteins. This protein has a molecular weight of approximately 27 kDa. Researchers and scientists often express GFP in various organisms as a luminescent tag helping them observe protein expression localization and interaction within cells. GFP tagging involves the fusion of GFP to a protein of interest enabling the study of the protein's function and dynamics without affecting the host cell.
Biological function summary
GFP serves as a marker due to its ability to emit green fluorescence without requiring additional substrates or cofactors. GFP does not function within complexes like other proteins but acts as a standalone tool to monitor physiological processes. Scientists utilize techniques such as Western blot ELISA and microscopy along with GFP to track and quantify proteins inside living cells. Anti-GFP antibodies can detect GFP fusion proteins in various applications providing valuable insights into protein behavior and allowing robust assays involving GFP.
Pathways
GFP itself does not participate actively in traditional biochemical or signaling pathways. Instead it enables visual tracking within pathways. Researchers utilize GFP to study pathways like MAPK/ERK and PI3K/AKT where they track proteins related to these pathways using GFP tagging. For instance fusing GFP with proteins like ERK1/2 allows tracking phosphorylation events and signal transduction in living cells leading to better understanding of cellular responses to different stimuli.
Researchers use GFP as a model to study gene expression and protein interactions under disease conditions. For example in neurological disorders GFP helps visualize neuronal pathways and protein aggregation processes. By tagging proteins such as amyloid precursor protein (APP) or tau with GFP scientists can study their role in Alzheimer's disease progression. Similarly GFP facilitates the investigation of cancer pathways allowing visualization of tumor-related proteins and helping researchers study how cancer cells grow and invade tissues supporting cancer research and therapy development.
