Description
Key facts
Detection method:Colorimetric,
Sample types:Urine, Plasma, Cell culture extracts, Tissue, Serum,
Assay type:Competitive,
Sensitivity:= 3 pg/mL,
Range:0.01 - 1000 ng/mL,
Assay Platform:Microplate
Product details:
Abcam's 14,15 EET / DHET
in vitro
ELISA kit is designed for accurate quantitative measurement in serum, plasma, urine, cell culture, tissue samples following proper isolation and purification.
A 96-well plate has been precoated with 14,15-DHET antibody. Samples and the 14,15-DHET-HRP conjugate are added to the wells, where 14,15-DHETin the sample competes with the added 14,15‑DHET-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of 14,15‑DHET in the sample and the intensity is measured at 450 nm.
It is well known that arachidonic acid (AA) will be converted to EET by P450 arachidonic acid epoxygenase (AA epoxygenase) and EET will be converted to DHET by soluble epoxide hydrolase (sEH) in vivo. Cytochrome P450 2J2 (CYP2J2) is a predominant human AA epoxygenase that produces all four EETs. In human carcinoma cells, rAAV-mediated over expression of CYP2J2 resulted in a marked increase in 14,15-DHET level in cell plasma, whereas rAAV-anti2J2-mediated silence of CYP2J2 expression significantly decreased its production. Abcam's14,15EET/DHET in vitro ELISA kit can be used to measure EET levels in cultured cells which express sEH.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
1415-EET also known as 1415-epoxyeicosatrienoic acid is a cytochrome P450-mediated metabolite of arachidonic acid. It belongs to the eicosanoid family of molecules and plays significant roles in the human body. 1415-EET expresses primarily in endothelial cells where it contributes to various physiological processes. The molecular weight of 1415-EET is approximately 306.5 g/mol and it operates as a signaling lipid that interacts with various proteins and enzymes to exert its effects.
Biological function summary
1415-EET behaves as a potent vasodilator and has anti-inflammatory properties making it essential in cardiovascular health. It forms part of a signaling complex that modulates vascular tone and cellular responses to stress. In addition to its role in vasodilation 1415-EET also affects ion channel activity and has been observed to inhibit platelet aggregation contributing to its protective functions in the vascular system.
Pathways
1415-EET participates in the cytochrome P450 epoxygenase pathway. This pathway metabolizes arachidonic acid into several EET isomers including 1415-EET. Related proteins such as soluble epoxide hydrolase (sEH) regulate the conversion of EETs to their less active dihydroxyeicosatrienoic acids (DHETs). EETs including 1415-EET interact with the PI3K/Akt signaling pathway which is vital for cellular proliferation and survival demonstrating their role in broader cellular processes.
1415-EET links to cardiovascular disorders and hypertension. Elevated levels of soluble epoxide hydrolase which degrades EETs associate with increased risks of hypertension due to the loss of EETs' vasodilatory properties. By modulating the levels of 1415-EET therapeutic interventions may target cardiovascular diseases highlighting its potential role in pharmacological developments. Similarly studies suggest roles in metabolic syndrome with the balance of EETs and their hydrolase playing a part in disease manifestation.
