Description
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Heparin Plasma, Other biological fluids, Serum,
Assay type:Indirect,
Sensitivity:= 98.6 %,
Assay Platform:Microplate
Product details:
Abcam's anti- Borrelia burgdorferi IgG Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Borrelia burgdorferi in Human serum, plasma and CSF.
A 96-well plate has been precoated with Borrelia burgdorferi antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Borrelia burgdorferi antigens. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Borrelia burgdorferi IgG sample captured in plate.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Borrelia burgdorferi IgG is an immunoglobulin subtype that targets the Borrelia burgdorferi bacterium a spirochete responsible for Lyme disease. The mass of IgG antibodies is approximately 150 kDa. These antibodies are produced by B-cells as a result of immune response and can be detected in serum. Borrelia burgdorferi IgG antibodies serve as important biomarkers in diagnostic assays including the ELISA Lyme tests and Western blots which are standard for confirming Lyme disease exposure.
Biological function summary
Borrelia burgdorferi IgG antibodies play a significant role in the adaptive immune system identifying and neutralizing foreign pathogens such as Borrelia burgdorferi. They form part of a complex immune response where the antibodies bind to specific antigens on the surface of the bacterium. This binding signals other components of the immune system to remove or destroy the pathogen. These antibodies result from previous infection indicating an ongoing or past exposure to the bacterial agent.
Pathways
Borrelia burgdorferi IgG has implications in immune system pathways like the humoral immune response. During this response B-cells differentiate into plasma cells that produce antibodies such as IgG. This process involves collaboration with T-helper cells which play a part in activating B-cells. The complement system a group of proteins also interacts with IgG to enhance pathogen destruction illustrating IgG's integration into immune regulation and pathogen elimination pathways.
Borrelia burgdorferi IgG holds a pivotal association with Lyme disease an infectious condition resulting from the bacterium's transmission via tick bites. The presence of Borrelia burgdorferi IgG in patient serum is a diagnostic indicator of Lyme disease especially in chronic cases. Moreover the antibody may also relate to post-treatment Lyme disease syndrome where symptoms continue after treatment completion. This connection emphasizes the involvement of Borrelia burgdorferi IgG in long-term immune processes and potential autoimmunity alongside proteins such as cytokines which may mediate inflammation in prolonged cases of the disease.
