Description
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Indirect,
Sensitivity:> 95 %,
Assay Platform:Microplate
Product details:
Abcam's anti-Parvovirus B 19 IgM Human
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Parvovirus B 19 in Human serum and plasma.
A 96-well plate has been precoated with Parvovirus B 19 antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Parvovirus B 19 antigens. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Parvovirus B 19 IgM sample captured in plate.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Parvovirus B19 IgM typically called Parvovirus IgM represents the immunoglobulin M antibodies that the human body produces in response to Parvovirus B19 infection. This IgM has an important role during the early stages of infection and aids in identifying recent exposure to the virus. Parvovirus B19 is a small single-stranded DNA virus with a mass approximately 5-6 kDa. The expression of IgM antibodies occurs mostly within the bloodstream shortly after infection. Detection of these antibodies is commonly performed using diagnostic assays such as the Parvo ELISA test and Parvo IgM ELISA kit.
Biological function summary
The detection of Parvovirus B19 IgM indicates the body's activation of the immune response against Parvovirus B19. This response serves as the body's mechanism to eliminate the virus and prevent its replication. IgM is not part of a complex but plays an important stand-alone role in a host's immune defense. Its presence also aids in identifying other immune system activity linking to additional responses such as the production of immunoglobulin G (IgG) later in the infection phase.
Pathways
Parvovirus B19 IgM links to the immune response pathway particularly in the production and maturation of antibody responses. It interacts with other antibodies and immune complexes present during a virus infection aiding in directing B cell activation and eventual IgG formation. No direct protein-protein interaction occurs with Parvovirus B19 specific IgM within these pathways however its presence assists in shaping further immune activities critical for viral clearance.
Parvovirus B19 IgM serves as a significant marker for conditions like erythema infectiosum also known as fifth disease and transient aplastic crisis in individuals with hemolytic disorders. The presence of IgM antibodies indicates an active or recent infection. Parvovirus B19 plays an important role in these conditions with its viral proteins interacting indirectly with the host's blood cell production mechanisms potentially leading to complications in cases of anemia or compromised immune systems.
