Description
Key facts
Assay type:Polymer Multiplex
Product details:
The DoubleStain IHC kit (ab183271) is designed to use with user supplied goat and mouse antibodies to detect two distinct antigens on human tissue or cell samples. This kit has been tested in paraffin tissues, however it can also be used on frozen specimens and freshly prepared monolayer cell smears.
Double staining is a common method used in immunohistochemistry for the detection of two distinct antigens in a single tissue. Abcam's DoubleStain IHC Kit supplies two polymer enzyme conjugates: AP polymer anti-Goat IgG and HRP(AEC) polymer anti-Mouse IgG with two distinct substrates/chromogens, BCIP/NBT (Purple) and AEC (Red). Users will apply the two enzyme conjugates onto the specimen sequentially. If only the anti-goat antigen is present only the BCIP/NBT(Purple) will be present and if the mouse antigen is present only the AEC(Red) will be present. Abcam's DoubleStain IHC Kit is a non-biotin system avoiding endogenous biotin non-specific binding.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
