Description
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, EDTA Plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 5.28 ng/mL,
Range:18.75 - 1200 ng/mL,
Assay time:1h 30m,
Assay Platform:Microplate
Product details:
Human Apolipoprotein B ELISA Kit ab190806 is a rapid single-wash 90-min Sandwich ELISA to measure Human Apolipoprotein B in citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 5.28 ng/mL.
How the assay works
Human Apolipoprotein B SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Apolipoprotein B ELISA Kit ab190806 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Apolipoprotein B antibody [EPR18817-107] - BSA and Azide free (Capture)
ab252574
- Anti-Apolipoprotein B antibody [EPR18817-119] - BSA and Azide free (Detector)
ab252633
Apolipoproteins are proteins that bind lipids to form lipoproteins, which carry fats and cholesterol in the bloodstream. Apolipoproteins provide structural support to these lipoproteins, may act as ligands for specific cell surface receptors or may modulate the activity of metabolic enzymes that act on lipoproteins. A major class of apolipoproteins is ApoB, which is found in two forms: ApoB-100 (550 kDa) and ApoB-48 (264 kDa). ApoB-48 is made from the N-terminal 48% of the Apolipoprotein B-100 protein due to a post-transcriptional mRNA editing by the ApoB mRNA editing enzyme ApoBec1. ApoBec1 causes a C to U transition, replacing a CAA glutamine encoding codon for a UAA stop codon and generating ApoB-48. ApoBec1 is expressed in the intestine but not the liver, thus ApoB-48 is secreted by the intestine to become the building block of chylomicrons, while ApoB-100 is secreted by the liver to become a component of very low-density lipoproteins (VLDLs), intermediate-density lipoproteins (IDLs), and low-density lipoproteins (LDLs). Importantly, ApoB is also post-translationally palmitoylated; palmitoylation is necessary for proper assembly of lipoproteins (including proper assembly of the hydrophobic core). Furthermore, ApoB-100 operates on the surface of LDLs to allow binding to the LDL receptor and transport for breakdown of LDLs.
Mutations in the
Apolipoprotein B
gene can cause several diseases. First, in familial hypobetalipoproteinemia (FHBL), mutations in the
Apolipoprotein B
gene cause production of abnormally short lengths of ApoB protein, lowering the amount of lipoproteins and hence decreasing the body's capacity to transport and absorb fat and fat-soluble vitamins. Second, in a form of inherited hypercholesterolemia called familial defective ApoB-100 (FDB), mutations in the
Apolipoprotein B
gene prevent LDLs from effectively binding to their receptors. As a result, fewer LDLs are removed from the blood, leading to hypercholesterolemia, distribution and buildup of cholesterol into tissues such as the skin and arteries, and an increased risk of coronary artery disease (CAD).
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Apolipoprotein B often referred to as ApoB is a core protein component of low-density lipoproteins (LDL) and is essential for their assembly and secretion. ApoB is also known as apolipoproteine B and apolipoprotein B protein. This protein has a large molecular weight approximately 550 kDa. ApoB is primarily expressed in the liver and to some extent in the intestine. Its large size allows it to provide structural integrity to lipoprotein particles playing a role in lipid metabolism.
Biological function summary
ApoB plays a significant role in lipid transport and metabolism. It is a critical component of the lipoprotein particle complex which is responsible for the transport of lipids such as cholesterol and triglycerides through the bloodstream. ApoB helps in the binding of lipoproteins to cell receptors facilitating the uptake of lipids by tissues. Its presence on the surface of lipoproteins makes it important for normal cholesterol homeostasis in the human body.
Pathways
ApoB is integrated within the lipid metabolism pathway. It interacts with proteins such as microsomal triglyceride transfer protein (MTP) during the assembly and secretion of lipoproteins. This pathway is essential for the normal transport of lipids throughout the body and influences processes like absorption of dietary fats. ApoB also plays a role in the pathways related to atherosclerosis where it influences the deposition of cholesterol in arterial walls.
ApoB is well-associated with cardiovascular diseases notably atherosclerosis and familial hypercholesterolemia. In these conditions elevated levels of ApoB correlate to an increased risk of plaque formation in blood vessels. ApoB in conjunction with LDL receptor and other related proteins influences lipid homeostasis and disorders where lipid oxidation or accumulation is present. Monitoring ApoB and its concentration can be vital for assessing cardiovascular disease risk.
