Description
Key facts
Assay time:25m,
Sample types:Tissue Lysate, Urine, Tissue Extracts, Whole Blood, Tissue Culture Media
Product details:
Deproteinizing Sample Preparation Kit - TCA (ab204708) offers a simple method to prepare samples for use in assays to measure small molecules, such as lipids, metabolites, metal ions etc.
This kit is useful where endogenous enzymes and other proteins would interfere with the assay, or would act directly to affect the level of the analyte to be measured, or would bind the analyte.
In this kit, trichloroacetic acid (TCA) is used to precipitate proteins by drastically lowering the sample pH. After removal of precipitated proteins, the pH of the sample is neutralized with neutralization buffer that is provided in the kit. Small molecules present in the sample can then be easily analyzed without interference from endogenous enzymes and other proteins .
The kit is easy to follow, convenient, and can be utilized for the preparation of a large number of samples in parallel. Samples prepared using this kit can be directly used in various bioassays.
This product is manufactured by BioVision, an Abcam company and was previously called K823 Deproteinizing Sample Preparation Kit II. K823-200 is the same size as the 200 test size of ab204708.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient, Storage information-Ambient
