Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Tissue, Suspension cells, Serum, Adherent cells,
Assay type:Enzyme activity (quantitative),
Sensitivity:< 20 µM,
Assay time:50m,
Assay Platform:Microplate reader
Product details:
How the assay works
In Acetate Assay Kit (Colorimetric) protocol, free acetate is converted to an intermediate in the presence of Acetate Enzyme Mix and Acetate Substrate Mix. The intermediate reduces the probe to a colored product with strong absorbance at OD=450 nm.
Acetate assay protocol summary:
- Add samples and standards to wells
- Add reaction mix
- Incubate for 40 min
- Analyze with microplate reader
How other researchers are using
Acetate Assay Kit (Colorimetric) has been used in a variety of sample type including:
- E. coli and Enterobacter supernatant
- Mouse spinal cord and serum samples
- Human Esophageal squamous cell carcinoma cell lines
References:
1-Estrela S al. 2022
2-Jin S et al. 2021
3-Odera J et al. 2020
This product is manufactured by BioVision, an Abcam company and was previously called K658 Acetate Colorimetric Assay Kit. K658-100 is the same size as the 100 test size of ab204719.
Acetate is an important component for biosynthesis. In living organisms, acetylation/deacetylation of proteins is critical for posttranslational regulation of their functions. When bound to CoA, Acetyl-CoA is central to metabolism of carbohydrates and fats. It is also involved in the synthesis of biogenic compounds such as neurotransmitter acetylcholine.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Acetate also known as acétate or acetae refers to the conjugate base of acetic acid and carries a molecular weight of approximately 59.04 g/mol. It is ubiquitously expressed across various tissues and cellular compartments playing a role in many metabolic processes. Acetate is produced primarily in the liver and is also present in the bloodstream contributing to its availability across diverse biological systems. Its ubiquitous distribution highlights its versatile role in cellular functions where it contributes as a substrate for biosynthesis.
Biological function summary
Many metabolic processes utilize acetate serving as an important building block in the synthesis of fatty acids and cholesterol. It is not typically associated with a complex but it collaborates with coenzymes and enzymes to drive biosynthetic reactions. For instance acetate acts as a precursor in the formation of acetyl-CoA an essential molecule in energy production and lipid synthesis. The convergence of acetate in such biochemical processes highlights its importance in maintaining cellular energy balance and material synthesis.
Pathways
Acetate prominently features in the acetyl-CoA biosynthesis pathway an important conduit for energy production. This pathway integrates acetate into cycles like the citric acid cycle where it partners with enzymes like citrate synthase. Another significant connection exists with the lipid biosynthesis pathway where acetate is converted into fatty acids relating closely with acetyl-CoA carboxylase. These pathways highlight the centrality of acetate in both energy provision and cell membrane synthesis affirming its integral role in cell metabolism.
Acetate influences metabolic conditions such as diabetes and obesity due to its role in lipid biosynthesis and energy metabolism. These conditions relate to alterations in acetate metabolism often involving dysregulation of acetyl-CoA levels. Additionally acetate is associated with cancer proliferation where increased acetate metabolism supports the rapid growth of cancer cells. The involvement of enzymes like acetyl-CoA synthetase 2 in these diseases underlines the enzymatic control of acetate's role in pathological states showing its broader impact on health and disease.
