Description
Key facts
Detection method:Fluorescent,
Sample types:Urine, Plasma,
Assay type:Quantitative,
Assay time:15m,
Assay Platform:Microplate reader
Product details:
How the assay works
In Adenosine Assay Kit (Fluorometric), adenosine is measured using adenosine deaminase followed by a multi-step enzymatic approach resulting in the generation of an intermediate that reacts with the adenosine probe, leading to the formation of a fluorescent product. The fluorescent product can be detected at Ex/Em = 535/587 nm, and its intensity is proportional to the amount of adenosine in the sample.
Adenosine assay protocol summary:
- Add samples and standards to wells
- Add reaction mix
- Incubate for 60 min at 37°C
- Analyze with fluorescence microplate reader (Ex/Em = 535/587 nm)
How other researchers are using
Adenosine Assay Kit (Fluorometric) has been used in a variety of sample type including:
- Mouse immortalized microglial cell line BV-2
- Mouse bronchoalveolar lavage (BAL) fluid
- Mouse Splenic CD4+CD25+ Tregs or CD4+CD25- T cells
References:
1-Tscherrig V al. 2023
2 Kelestemur T et al. 2023
3-Kong L et al. 2023
This product is manufactured by BioVision, an Abcam company and was previously called K327 Adenosine Assay Kit (Fluorometric). K327-100 is the same size as the 100 test size of ab211094.
Adenosine, a purine nucleoside, is present throughout the body. It plays an important role in energy transfer via the formation of ATP, ADP and AMP and in signal transduction via the formation of cAMP. Adenosine mediates its effects directly via adenosine receptors A1, A2A, A2B and A3. It regulates myocardial oxygen consumption and coronary blood flow, exerts anti-inflammatory effects throughout the body and also regulates the Renin-Angiotensin system. It also plays a role in tissue damage and repair, and cell death. Plasma adenosine levels are increased in patients with ischemic and non-ischemic heart failure.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Adenosine a nucleoside composed of adenine attached to a ribose sugar plays an important role as a signaling molecule in the body. It has a molecular mass of approximately 267.24 g/mol. Although found throughout the body adenosine concentrations are especially high in the brain and heart tissues where they facilitate vital communication processes. When studying adenosine researchers often use specific tools like the adenosine assay kit and adenosine ELISA to measure and analyze its levels in various tissues and fluids.
Biological function summary
Adenosine influences a wide range of physiological processes by interacting with specific receptors. These receptors known as P1 receptors include A1 A2A A2B and A3 with each having unique tissue distribution and function. Adenosine functions as part of the adenosine receptor complex impacting cellular energy transfer and serving as an inhibitor in the central nervous system. It modulates neurotransmitter release and influences heart rate by acting on the sinoatrial node therefore playing a significant role in sleep regulation and cardiac function.
Pathways
Adenosine operates in key physiological pathways like the purinergic signaling and cAMP pathways. In the purinergic signaling pathway adenosine acts as a neuromodulator and regulates immune responses. Through interactions with protein kinases adenosine influences the cAMP signaling pathway by controlling processes such as ATP conversion. Related proteins in these pathways include adenosine deaminase which aids in the breakdown of adenosine and the various adenosine receptors which mediate its signaling effects.
Elevated adenosine levels have links to cardiovascular and neurological conditions. In cardiovascular diseases adenosine interacts with G protein-coupled receptors modulating heart rate and protecting against ischemia. In the case of neurological disorders adenosine has been connected to conditions such as epilepsy where altered adenosine metabolism affects neural excitability. Adenosine's role in both of these contexts highlights its significance in maintaining homeostasis and emphasizes the importance of its regulation within these pathways.
