Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Serum,
Reacts with:Human,
Assay type:Indirect,
Sensitivity:= 92.6 %,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Abcam's Anti-Schistosoma mansoni IgM Human ELISA Kit is an
in vitro
enzyme linked immunosorbent assay (ELISA) (ab211773) for the qualitative determination of IgM antibodies against Schistosoma mansoni in human serum or plasma (citrate or heparin).
Microplates are precoated with Schistosoma mansoni antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material, horseradish peroxidase (HRP) labelled anti-human IgM conjugate is added. This conjugate binds to the captured Schistosoma mansoni -specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Schistosoma mansoni -specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.
Schistosomes belong to the class of distomas (trematodes). They rank among the most frequent pathogens. Estimations originate in more than 200 million affected people. The mature parasites are 6 – 22 mm long. The most important species are Schistosoma mansoni, S. japonicum and S. haematobium. Schistosoma mansoni is common in Africa, South America and Middle East.
Schistosomiasis (bilharziosis) is (depending on species and location of the parasites) a disease of the intestine, liver and spleen resp. urinary passages. Humans are (re)infected by contact with fresh water which is contaminated by ova containing urine or faeces. If larvae bore into human skin, first a transient skin reaction appears (itch with exanthema or erythema, by repeatedly infection cercarial dermatitis is possible). After 3 - 10 weeks the meanwhile sexually mature worms synthesize cytotoxic and allergic substances which course feverish reaction in humans (Katayama fever). The infected person is mostly harmed by the eggs, which get into organs via blood excreting proteins and glycoproteins. The person reacts under participation of own antibodies and immune complexes with formation of granuloma and granulomatous proliferation in intestine and urinary bladder mucosa. Not excreted eggs die after 3 weeks and will be dissolved or calcified. The affected tissue gets fibrous. In final stage bilharziosis will cause death.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Schistosoma mansoni IgM also known as immunoglobulin M specific to S. mansoni plays a role in the immune response against the Schistosoma mansoni parasite. This target present in the plasma of infected individuals typically forms as a pentamer and often appears early during infection. Its molecular mass in pentameric form generally exceeds 900 kDa. It is mostly produced by B cells located in lymphoid tissues.
Biological function summary
The Schistosoma mansoni IgM antibodies are important in the host's immune defense. The antibodies can neutralize antigens released by parasites acting as a critical first line of defense. As a part of the immune complex IgM can facilitate the activation of the classical complement pathway enhancing the clearance of parasites. It is not part of any larger complex directly but works closely with cellular and humoral components of the immune system.
Pathways
The Schistosoma mansoni IgM is mainly involved in the classical pathway of the complement system. This pathway helps in opsonization and lysis of pathogens. The IgM antibodies activate complement component proteins like C1q which play an important role in pathogen clearance. They have interactions with other immune-related proteins such as complement factors and other immunoglobulins which ensure a coordinated immune response.
Schistosoma mansoni IgM has links to bilharzia (schistosomiasis) a disease caused by Schistosoma parasites. The presence of Schistosoma mansoni IgM is used as an indicator of infection through ELISA serology a valuable diagnostic tool. During the ELISA test process a bilharzia test kit detects these antibodies marking a positive infection status. IgM's connection with other immune proteins helps target the parasites and mitigate symptoms related to this chronic condition.
