Abcam, ab220074-100µL, Alexa Fluor® 488 Anti-BrdU antibody [BU1/75 (ICR1)]

Product details
What is this antibody validated in? Alexa Fluor® 488 Anti-BrdU antibody [BU1/75 (ICR1)] (ab220074) is a rat monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF). Related products Antibody clone BU1/75 (ICR1) is also available pre-conjugated to a variety of labels for your convenience – Anti-BrdU Alexa Fluor® 488 [BU1/75 (ICR1)] (ab220074). Other related products We have a range of other formats of antibody clone [BU1/75 (ICR1)] also available for your convenience: ab6326, Alexa Fluor® 488 - ab220074, Alexa Fluor® 647 - ab220075, Alexa Fluor® 594 - ab220076, HRP - ab220507, Alexa Fluor® 555 - ab221240, Carrier free - ab264079
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].

Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Bromodeoxyuridine acts as a marker for identifying cells in the S-phase of the cell cycle. During this phase DNA synthesis occurs and BrdU incorporates into the replicating DNA strand. When used in combination with anti-BrdU antibodies it enables researchers to identify and quantify dividing cells. This capability makes BrdU incorporation a widely accepted method to study cell proliferation in various biological samples. It functions independently without forming larger complexes.
Pathways
Bromodeoxyuridine integration highlights its role in DNA replication and repair pathways. It helps in the study of cell growth regulation pathways where proteins like PCNA (Proliferating Cell Nuclear Antigen) are involved. BrdU pairing with anti-BrdU antibodies allows assessment of DNA synthesis therefore aiding in the investigation of critical pathways like the cell cycle regulation pathway. In this context BrdU kits provide a consistent method for assessing dynamic changes in proliferative states.