Description
Key facts
Detection method:Colorimetric,
Sample types:Cell culture media, Citrate plasma, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 47.34 pg/mL,
Range:109.38 - 7000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human ALT ELISA Kit ab234578 is a rapid single-wash 90-min ELISA kit to measure Human ALT in . This SimpleStep ELISA® sensitivity is 47.34 pg/mL.
How the assay works
Human ALT SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant human ALT protein in serum, plasma, and cell culture supernatant samples only. Tissue extract samples have not been tested with this kit. This kit is incompatible with plasma- heparin and cell extract samples.
Human ALT ELISA Kit ab234578 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human ALT ELISA Kit ab234578
Human ALT ELISA Kit ab234578 has been used to validate a human Liver-Chip as a model for predicting drug-induced liver injury
(1)(2)
and to study novel therapeutics to hepatitis B
References:
Daniel Levner et al. 2022, PMID: 36473994,
Janey Ronxhi et al. 2022, PMID: 35790095,
Sung-Gyoo Park, et al. 2019, PMID: 31097716
Save your precious samples
Our 384-well Human ALT ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human ALT Antibody Pair - BSA and Azide free
ab244121
- Anti-Alanine Transaminase antibody [EPR21068-102] - BSA and Azide free (Capture)
ab244765
- Anti-Alanine Transaminase antibody [EPR21068-86] - BSA and Azide free (Detector)
ab245036
Related and recommended products
Human ALT ELISA kit ab234578 is commonly used by researchers to measure liver function.
Other ELISA kits to measure liver function include:
- Human AST ELISA kit
ab263881
- Human Albumin ELISA kit
ab179887
- Albumin (BCG) Assay Kit (Colorimetric)
ab235628
These ELISA kits are also available for Mouse/Rat:
- Mouse ALT ELISA kit
ab282882
- Mouse AST ELISA kit
ab263882
- Mouse Albumin ELISA kit
ab207620
- Rat ALT ELISA kit abab234579
- Rat AST ELISA kit
ab263883
- Rat Albumin ELISA kit
ab235642
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alanine transaminase also known as ALT or alanine aminotransferase is an important enzyme in the body. It has a molecular mass of approximately 54 kDa and catalyzes the conversion between alanine and pyruvate by transferring an amino group. ALT is mainly expressed in the liver but also present in other tissues like the heart and muscles. It is often measured as 'ALT activity' in clinical settings to ascertain liver health. In laboratories an 'ALT assay' or 'ALT kit' can determine alanine transaminase activity.
Biological function summary
Alanine transaminase plays a critical role in amino acid metabolism and energy production. It facilitates the transamination process which is significant for the balance of amino acids and carbohydrates. ALT as a standalone enzyme does not form part of a protein complex but works closely with alanine aminotransferase reactions. It helps maintain the cellular nitrogen balance linking nitrogen metabolism with energy pathways.
Pathways
The role of alanine transaminase is to interconnect carbohydrate and amino acid metabolism. It participates in the gluconeogenesis pathway and the citric acid cycle important for energy production. Through its activity in these pathways it interacts with proteins like aspartate aminotransferase (AST) affecting the balance of amino and keto acids in cells.
Alanine transaminase levels indicate liver conditions such as hepatitis and liver cirrhosis. Elevated ALT activity suggests liver damage or inflammation. It also connects to conditions involving muscular injury. In these scenarios increased presence and activity of AST often accompany ALT providing a broader picture of the extent of tissue damage. Understanding the involvement of ALT in these pathways helps diagnose and monitor the progression of relevant health issues.
