Description
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate, Cell culture media, Plasma, Tissue Extracts, Urine,
Assay type:Quantitative,
Sensitivity:= 0.1 nM
Product details:
cAMP Assay Kit (Competitive ELISA) ab234585 is a competitive ELISA to measure cAMP Assay Kit (Competitive ELISA) in plasma, urine, cell culture media, cell lysate, tissue extracts with a sensitivity of 0.1 nM.
How the assay works:
Colorimetric ELISA cAMP Assay Kit (ab234585) is based on the competition between HRP-labeled cAMP and free cAMP for a fixed number of cAMP antibody binding sites. HRP-cAMP is displaced from the HRP-cAMP/anti-cAMP antibody complex by unlabeled free cAMP. In the absence of cAMP, HRP-cAMP conjugate is bound to anti-cAMP antibody exclusively. However, the unlabeled free cAMP in the test samples competes for anti-cAMP antibody with the HRPcAMP antibody conjugate, therefore inhibits the binding of HRPcAMP to anti-cAMP antibody. The kit provides the sensitive method for detecting adenylate cyclase activity in biochemical or cellbased assay system. Compared to other ELISA cAMP assay kits, our kit eliminates the tedious acetylation step, and provides the readyto-use Anti-cAMP Ab coated 96-well plate and HRP substrate Green Probe to quantify the HRP activity. The color product formed is proportional to the activity of HRP-cAMP.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
cAMP Assay Kit (Competitive ELISA) ab234585 protocol summary
1. Add cAMP standard or test samples into the anti-cAMP coated 96-well plate and incubate at room temperature for 5-10 minutes
2. Add 1X HRP-cAMP conjugate and Incubate at room temperature for 3 hours
3. Wash wells 4 times with Washing Buffer. Add Green Probe and incubate at room temperature for 1 to 3 hours
4. Monitor absorbance increase at 405, 650 or 740 nm.
If you are using fluorometric detection, we recommend cAMP Assay Kit (Fluorometric) (ab138880)
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
