Description
Key facts
Detection method:Colorimetric,
Sample types:Serum,
Assay type:Quantitative,
Sensitivity:= 5 µg,
Assay Platform:Microplate reader
Product details:
Albumin (BCG) Assay Kit (Colorimetric) (ab235628) is a simple high-throughput assay that detects Albumin concentration in serum.
Albumin BCG assay method
The BCG albumin assay method is based on the selective interaction between Bromocresol Green (BCG) and albumin forming a chromophore that can be detected at 620 nm. The signal is directly proportional to the amount of albumin present in the serum. BCG does not react with other abundant plasma proteins like IgG.
The assay can detect as low as 5 μg (0.01 g/dL) of albumin in serum samples.
Albumin BCG assay protocol summary
- add undiluted serum to 96-well plate
- add assay buffer to adjust final volume to 50 μL/well
- add reaction mix
- incubate for 20-min
- analyze on plate reader
Other notes
This product was previously called K554 Biovison Albumin (BCG) Assay Kit (Colorimetric). Biovision was acquired by Abcam in 2021.
Albumin is the most abundant protein in human blood and is highly conserved among vertebrates. It plays a pivotal physiological role in maintenance of plasma osmotic pressure, vascular permeability, and transport of cholesterol, bile pigments, nitric oxide, metals, and other small molecules in the body. It also functions as a free radical scavenger of reactive oxygen and nitrogen species, triggers cell signaling processes, possesses anti-inflammatory and coagulatory effects.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.
Biological function summary
Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.
Pathways
More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.
Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.
